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X-Chromosome Inactivation

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Cover of 'X-Chromosome Inactivation'

Table of Contents

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    Book Overview
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    Chapter 1 Screening for Factors Involved in X Chromosome Inactivation Using Haploid ESCs
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    Chapter 2 Unbiased Genetic Screen to Identify Factors Involved in X-Chromosome Inactivation Using a Pooled Bar-Coded shRNA Library
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    Chapter 3 ChIRP-MS: RNA-Directed Proteomic Discovery
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    Chapter 4 Manipulation of Xist Imprinting in Mouse Preimplantation Embryos
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    Chapter 5 Somatic Cell Nuclear Transfer in Mice: Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status
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    Chapter 6 Live Imaging of Xist RNA
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    Chapter 7 Live Imaging of X-Chromosome Inactivation and Reactivation Kinetics
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    Chapter 8 Visualizing the Dynamics of Inactive X Chromosomes in Living Cells Using Antibody-Based Fluorescent Probes
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    Chapter 9 Visualization of Hidden Epitopes at the Inactive X Chromosome
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    Chapter 10 Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation
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    Chapter 11 Simultaneous RNA–DNA FISH in Mouse Preimplantation Embryos
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    Chapter 12 Combined Immunofluorescence, RNA FISH, and DNA FISH in Preimplantation Mouse Embryos
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    Chapter 13 RNA-FISH and Immunofluorescence of Mouse Preimplantation and Postimplantation Embryos
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    Chapter 14 Experimental Analysis of Imprinted Mouse X-Chromosome Inactivation
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    Chapter 15 X-Chromosome Inactivation and Escape from X Inactivation in Mouse
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    Chapter 16 Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles
Attention for Chapter 9: Visualization of Hidden Epitopes at the Inactive X Chromosome
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Chapter title
Visualization of Hidden Epitopes at the Inactive X Chromosome
Chapter number 9
Book title
X-Chromosome Inactivation
Published in
Methods in molecular biology, September 2018
DOI 10.1007/978-1-4939-8766-5_9
Pubmed ID
Book ISBNs
978-1-4939-8765-8, 978-1-4939-8766-5
Authors

Emma R. Stewart, Dawn Coverley, Stewart, Emma R., Coverley, Dawn

Abstract

Immunodetection of nuclear antigens is often complicated by epitope masking, so that proteins known to function in the nucleus are sometimes not easily detected at their sites of action. Moreover, protein populations that are detected before unmasking can be very different to those seen after removal of nucleic acids. This is particularly true for components of the nuclear matrix, including those known to function at the inactive X chromosome. Here we describe an unmasking protocol that reveals previously undetected proteins at the inactive X chromosome in mouse fibroblasts.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 29%
Professor 1 14%
Student > Ph. D. Student 1 14%
Student > Doctoral Student 1 14%
Researcher 1 14%
Other 0 0%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Agricultural and Biological Sciences 2 29%
Immunology and Microbiology 1 14%
Medicine and Dentistry 1 14%
Unknown 1 14%