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Chapter title |
Visualization of Hidden Epitopes at the Inactive X Chromosome
|
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Chapter number | 9 |
Book title |
X-Chromosome Inactivation
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Published in |
Methods in molecular biology, September 2018
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DOI | 10.1007/978-1-4939-8766-5_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8765-8, 978-1-4939-8766-5
|
Authors |
Emma R. Stewart, Dawn Coverley, Stewart, Emma R., Coverley, Dawn |
Abstract |
Immunodetection of nuclear antigens is often complicated by epitope masking, so that proteins known to function in the nucleus are sometimes not easily detected at their sites of action. Moreover, protein populations that are detected before unmasking can be very different to those seen after removal of nucleic acids. This is particularly true for components of the nuclear matrix, including those known to function at the inactive X chromosome. Here we describe an unmasking protocol that reveals previously undetected proteins at the inactive X chromosome in mouse fibroblasts. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 29% |
Professor | 1 | 14% |
Student > Ph. D. Student | 1 | 14% |
Student > Doctoral Student | 1 | 14% |
Researcher | 1 | 14% |
Other | 0 | 0% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Agricultural and Biological Sciences | 2 | 29% |
Immunology and Microbiology | 1 | 14% |
Medicine and Dentistry | 1 | 14% |
Unknown | 1 | 14% |