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X-Chromosome Inactivation

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Cover of 'X-Chromosome Inactivation'

Table of Contents

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    Book Overview
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    Chapter 1 Screening for Factors Involved in X Chromosome Inactivation Using Haploid ESCs
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    Chapter 2 Unbiased Genetic Screen to Identify Factors Involved in X-Chromosome Inactivation Using a Pooled Bar-Coded shRNA Library
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    Chapter 3 ChIRP-MS: RNA-Directed Proteomic Discovery
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    Chapter 4 Manipulation of Xist Imprinting in Mouse Preimplantation Embryos
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    Chapter 5 Somatic Cell Nuclear Transfer in Mice: Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status
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    Chapter 6 Live Imaging of Xist RNA
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    Chapter 7 Live Imaging of X-Chromosome Inactivation and Reactivation Kinetics
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    Chapter 8 Visualizing the Dynamics of Inactive X Chromosomes in Living Cells Using Antibody-Based Fluorescent Probes
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    Chapter 9 Visualization of Hidden Epitopes at the Inactive X Chromosome
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    Chapter 10 Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation
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    Chapter 11 Simultaneous RNA–DNA FISH in Mouse Preimplantation Embryos
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    Chapter 12 Combined Immunofluorescence, RNA FISH, and DNA FISH in Preimplantation Mouse Embryos
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    Chapter 13 RNA-FISH and Immunofluorescence of Mouse Preimplantation and Postimplantation Embryos
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    Chapter 14 Experimental Analysis of Imprinted Mouse X-Chromosome Inactivation
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    Chapter 15 X-Chromosome Inactivation and Escape from X Inactivation in Mouse
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    Chapter 16 Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles
Attention for Chapter 12: Combined Immunofluorescence, RNA FISH, and DNA FISH in Preimplantation Mouse Embryos
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Chapter title
Combined Immunofluorescence, RNA FISH, and DNA FISH in Preimplantation Mouse Embryos
Chapter number 12
Book title
X-Chromosome Inactivation
Published in
Methods in molecular biology, September 2018
DOI 10.1007/978-1-4939-8766-5_12
Pubmed ID
Book ISBNs
978-1-4939-8765-8, 978-1-4939-8766-5
Authors

Ikuhiro Okamoto, Okamoto, Ikuhiro

Abstract

Transcriptional and epigenetic dynamics of the genome occur during early development in mammals. It has been difficult to study these dynamics due to the limitation of materials and the difficulty of handling. In this chapter, we describe our attempt to apply a combination of immunofluorescence (IF), and RNA and DNA fluorescent in situ hybridization (FISH) in preimplantation mouse embryos. We have concentrated on refining these techniques to study the dynamics of X chromosome inactivation in mouse embryos. The techniques and general underlying principles described here should be applicable to other mammals of interest.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 40%
Student > Doctoral Student 3 30%
Student > Ph. D. Student 1 10%
Student > Master 1 10%
Unknown 1 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 30%
Biochemistry, Genetics and Molecular Biology 2 20%
Immunology and Microbiology 2 20%
Medicine and Dentistry 2 20%
Unknown 1 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 September 2018.
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#20,533,292
of 23,103,436 outputs
Outputs from Methods in molecular biology
#9,978
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Outputs of similar age
#294,089
of 337,900 outputs
Outputs of similar age from Methods in molecular biology
#188
of 248 outputs
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