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Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers

Overview of attention for article published in PLOS ONE, December 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • Good Attention Score compared to outputs of the same age and source (76th percentile)

Mentioned by

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1 news outlet

Citations

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17 Dimensions

Readers on

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36 Mendeley
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Title
Delivery of a Chlamydial Adhesin N-PmpC Subunit Vaccine to the Ocular Mucosa Using Particulate Carriers
Published in
PLOS ONE, December 2015
DOI 10.1371/journal.pone.0144380
Pubmed ID
Authors

Aleksandra Inic-Kanada, Marijana Stojanovic, Simone Schlacher, Elisabeth Stein, Sandra Belij-Rammerstorfer, Emilija Marinkovic, Ivana Lukic, Jacqueline Montanaro, Nadine Schuerer, Nora Bintner, Vesna Kovacevic-Jovanovic, Ognjen Krnjaja, Ulrike Beate Mayr, Werner Lubitz, Talin Barisani-Asenbauer

Abstract

Trachoma, caused by the intracellular bacterium Chlamydia trachomatis (Ct), remains the world's leading preventable infectious cause of blindness. Recent attempts to develop effective vaccines rely on modified chlamydial antigen delivery platforms. As the mechanisms engaged in the pathology of the disease are not fully understood, designing a subunit vaccine specific to chlamydial antigens could improve safety for human use. We propose the delivery of chlamydia-specific antigens to the ocular mucosa using particulate carriers, bacterial ghosts (BGs). We therefore characterized humoral and cellular immune responses after conjunctival and subcutaneous immunization with a N-terminal portion (amino acid 1-893) of the chlamydial polymorphic membrane protein C (PmpC) of Ct serovar B, expressed in probiotic Escherichia coli Nissle 1917 bacterial ghosts (EcN BGs) in BALB/c mice. Three immunizations were performed at two-week intervals, and the immune responses were evaluated two weeks after the final immunization in mice. In a guinea pig model of ocular infection animals were immunized in the same manner as the mice, and protection against challenge was assessed two weeks after the last immunization. N-PmpC was successfully expressed within BGs and delivery to the ocular mucosa was well tolerated without signs of inflammation. N-PmpC-specific mucosal IgA levels in tears yielded significantly increased levels in the group immunized via the conjunctiva compared with the subcutaneously immunized mice. Immunization with N-PmpC EcN BGs via both immunization routes prompted the establishment of an N-PmpC-specific IFNγ immune response. Immunization via the conjunctiva resulted in a decrease in intensity of the transitional inflammatory reaction in conjunctiva of challenged guinea pigs compared with subcutaneously and non-immunized animals. The delivery of the chlamydial subunit vaccine to the ocular mucosa using a particulate carrier, such as BGs, induced both humoral and cellular immune responses. Further investigations are needed to improve the immunization scheme and dosage.

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 36 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 17%
Student > Master 6 17%
Student > Bachelor 4 11%
Student > Doctoral Student 3 8%
Professor > Associate Professor 3 8%
Other 3 8%
Unknown 11 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 17%
Immunology and Microbiology 5 14%
Medicine and Dentistry 4 11%
Nursing and Health Professions 3 8%
Agricultural and Biological Sciences 2 6%
Other 4 11%
Unknown 12 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 December 2015.
All research outputs
#3,708,917
of 20,585,116 outputs
Outputs from PLOS ONE
#46,735
of 177,664 outputs
Outputs of similar age
#73,810
of 398,152 outputs
Outputs of similar age from PLOS ONE
#1,089
of 4,851 outputs
Altmetric has tracked 20,585,116 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 177,664 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 14.4. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 398,152 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 4,851 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.