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The Ubiquitin Proteasome System

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Cover of 'The Ubiquitin Proteasome System'

Table of Contents

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    Book Overview
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    Chapter 1 Characterization of RING-Between-RING E3 Ubiquitin Transfer Mechanisms
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    Chapter 2 Single-Turnover RING/U-Box E3-Mediated Lysine Discharge Assays
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    Chapter 3 Methods for NAD-Dependent Ubiquitination Catalyzed by Legionella pneumophila Effector Proteins
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    Chapter 4 Using In Vitro Ubiquitylation Assays to Estimate the Affinities of Ubiquitin-Conjugating Enzymes for Their Ubiquitin Ligase Partners
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    Chapter 5 Competition Assay for Measuring Deubiquitinating Enzyme Substrate Affinity
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    Chapter 6 Enzymatic Assembly of Ubiquitin Chains
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    Chapter 7 Ubiquitin-Activated Interaction Traps (UBAITs): Tools for Capturing Protein-Protein Interactions
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    Chapter 8 Generating Intracellular Modulators of E3 Ligases and Deubiquitinases from Phage-Displayed Ubiquitin Variant Libraries
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    Chapter 9 Integrated Proteogenomic Approach for Identifying Degradation Motifs in Eukaryotic Cells
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    Chapter 10 A Method to Monitor Protein Turnover by Flow Cytometry and to Screen for Factors that Control Degradation by Fluorescence-Activated Cell Sorting
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    Chapter 11 E. coli-Based Selection and Expression Systems for Discovery, Characterization, and Purification of Ubiquitylated Proteins
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    Chapter 12 Strategies to Trap Enzyme-Substrate Complexes that Mimic Michaelis Intermediates During E3-Mediated Ubiquitin-Like Protein Ligation
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    Chapter 13 Small-Angle X-Ray Scattering for the Study of Proteins in the Ubiquitin Pathway
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    Chapter 14 Methods for Preparing Cryo-EM Grids of Large Macromolecular Complexes
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    Chapter 15 Recombinant Expression, Unnatural Amino Acid Incorporation, and Site-Specific Labeling of 26S Proteasomal Subcomplexes
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    Chapter 16 Native Gel Approaches in Studying Proteasome Assembly and Chaperones
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    Chapter 17 Measuring the Overall Rate of Protein Breakdown in Cells and the Contributions of the Ubiquitin-Proteasome and Autophagy-Lysosomal Pathways
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    Chapter 18 Methods to Rapidly Prepare Mammalian 26S Proteasomes for Biochemical Analysis
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    Chapter 19 Measurement of the Multiple Activities of 26S Proteasomes
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    Chapter 20 Exploring the Regulation of Proteasome Function by Subunit Phosphorylation
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    Chapter 21 Scalable In Vitro Proteasome Activity Assay
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    Chapter 22 Exploring the Rampant Expansion of Ubiquitin Proteomics
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    Chapter 23 Ubiquitin diGLY Proteomics as an Approach to Identify and Quantify the Ubiquitin-Modified Proteome
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    Chapter 24 Interpreting the Language of Polyubiquitin with Linkage-Specific Antibodies and Mass Spectrometry
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    Chapter 25 Dissecting Dynamic and Heterogeneous Proteasome Complexes Using In Vivo Cross-Linking-Assisted Affinity Purification and Mass Spectrometry
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The Ubiquitin Proteasome System
Published by
Springer New York, January 2018
DOI 10.1007/978-1-4939-8706-1
978-1-4939-8705-4, 978-1-4939-8706-1

Thibault Mayor, Gary Kleiger

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Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 100%