Chapter title |
Interpreting the Language of Polyubiquitin with Linkage-Specific Antibodies and Mass Spectrometry
|
---|---|
Chapter number | 24 |
Book title |
The Ubiquitin Proteasome System
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8706-1_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8705-4, 978-1-4939-8706-1
|
Authors |
Marissa L. Matsumoto, Erick R. Castellanos, Yi Jimmy Zeng, Donald S. Kirkpatrick, Matsumoto, Marissa L., Castellanos, Erick R., Zeng, Yi Jimmy, Kirkpatrick, Donald S. |
Abstract |
Posttranslational modification of cellular proteins by ubiquitin serves a variety of functions. Among the multitude of ubiquitin substrates, ubiquitin itself is the most prevalent. For many years, the direct detection of polyubiquitin chains attached to cellular substrates was not practical, with cell biologists relegated to indirect approaches involving site-directed mutagenesis or in vitro biochemistry. Recent advances in two technologies-polyubiquitin linkage-specific antibodies and mass spectrometry proteomics, have overcome that limitation. Using one or both of these, the direct analysis of polyubiquitin chain linkages on cellular substrate proteins may be performed. This paper describes the complimentary nature of linkage-specific antibodies and mass spectrometry proteomics for the characterization of complex ubiquitin signals using lessons learned in early development of both technologies. |
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