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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Microglia enhanced the angiogenesis, migration and proliferation of co-cultured RMECs
|
|---|---|
| Published in |
BMC Ophthalmology, September 2018
|
| DOI | 10.1186/s12886-018-0886-z |
| Pubmed ID | |
| Authors |
Xinyi Ding, Ruiping Gu, Meng Zhang, Hui Ren, Qinmeng Shu, Gezhi Xu, Haixiang Wu |
| Abstract |
Attention is increasingly being given to microglia-related inflammation in neovascular diseases, such as diabetic retinopathy and age-related macular disease. Evidence shows that activated microglia contribute to disruption of the blood-retinal barrier, however, the mechanism is unclear. In this study, we aimed to clarify whether and how microglia affect the function of retinal microvascular endothelial cells (RMECs). We activated microglia by Lipopolysaccharides (LPS) stimulation. After co-culturing static or activated microglia with RMECs using the Transwell system, we evaluated the function of RMECs. Vascular endothelial growth factor-A (VEGF-A) and platelet-derived growth factor-BB (PDGF-BB) levels in the supernatant from the lower chamber were evaluated by ELISA. Angiogenesis, migration, and proliferation of RMECs were assessed by tube formation, wound healing, and WST-1 assays. The expression levels of tight junction proteins (ZO-1 and occludin) and endothelial markers (CD31 and CD34) were examined by Western blot analysis. We successfully established an LPS-activated microglia model and co-culture system of static or activated microglia with RMECs. In the co-culture system, we showed that microglia, especially activated microglia stimulated VEGF-A and PDGF-BB expression, enhanced angiogenesis, migration, proliferation, and permeability, and altered the phenotype of co-cultured RMECs. Microglia, especially activated microglia, play important roles in angiogenesis and maintenance of vascular function hemostasis in the retinal microvasculature. The mechanism needs further investigation and clarification. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 27% |
| Student > Master | 4 | 11% |
| Student > Doctoral Student | 3 | 8% |
| Student > Postgraduate | 2 | 5% |
| Researcher | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 13 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 5 | 14% |
| Medicine and Dentistry | 4 | 11% |
| Biochemistry, Genetics and Molecular Biology | 4 | 11% |
| Agricultural and Biological Sciences | 2 | 5% |
| Psychology | 2 | 5% |
| Other | 5 | 14% |
| Unknown | 15 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 October 2022.
All research outputs
#6,675,049
of 23,575,882 outputs
Outputs from BMC Ophthalmology
#308
of 2,523 outputs
Outputs of similar age
#117,855
of 342,442 outputs
Outputs of similar age from BMC Ophthalmology
#4
of 33 outputs
Altmetric has tracked 23,575,882 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 2,523 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 342,442 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.