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Microglia enhanced the angiogenesis, migration and proliferation of co-cultured RMECs

Overview of attention for article published in BMC Ophthalmology, September 2018
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Title
Microglia enhanced the angiogenesis, migration and proliferation of co-cultured RMECs
Published in
BMC Ophthalmology, September 2018
DOI 10.1186/s12886-018-0886-z
Pubmed ID
Authors

Xinyi Ding, Ruiping Gu, Meng Zhang, Hui Ren, Qinmeng Shu, Gezhi Xu, Haixiang Wu

Abstract

Attention is increasingly being given to microglia-related inflammation in neovascular diseases, such as diabetic retinopathy and age-related macular disease. Evidence shows that activated microglia contribute to disruption of the blood-retinal barrier, however, the mechanism is unclear. In this study, we aimed to clarify whether and how microglia affect the function of retinal microvascular endothelial cells (RMECs). We activated microglia by Lipopolysaccharides (LPS) stimulation. After co-culturing static or activated microglia with RMECs using the Transwell system, we evaluated the function of RMECs. Vascular endothelial growth factor-A (VEGF-A) and platelet-derived growth factor-BB (PDGF-BB) levels in the supernatant from the lower chamber were evaluated by ELISA. Angiogenesis, migration, and proliferation of RMECs were assessed by tube formation, wound healing, and WST-1 assays. The expression levels of tight junction proteins (ZO-1 and occludin) and endothelial markers (CD31 and CD34) were examined by Western blot analysis. We successfully established an LPS-activated microglia model and co-culture system of static or activated microglia with RMECs. In the co-culture system, we showed that microglia, especially activated microglia stimulated VEGF-A and PDGF-BB expression, enhanced angiogenesis, migration, proliferation, and permeability, and altered the phenotype of co-cultured RMECs. Microglia, especially activated microglia, play important roles in angiogenesis and maintenance of vascular function hemostasis in the retinal microvasculature. The mechanism needs further investigation and clarification.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 10%
Unspecified 1 10%
Unknown 8 80%
Readers by discipline Count As %
Unspecified 1 10%
Neuroscience 1 10%
Unknown 8 80%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 September 2018.
All research outputs
#12,019,736
of 13,555,083 outputs
Outputs from BMC Ophthalmology
#598
of 814 outputs
Outputs of similar age
#228,343
of 264,839 outputs
Outputs of similar age from BMC Ophthalmology
#4
of 4 outputs
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So far Altmetric has tracked 814 research outputs from this source. They receive a mean Attention Score of 2.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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