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Spatially transformed fluorescence image data for ERK-MAPK and selected proteins within human epidermis

Overview of attention for article published in Giga Science, December 2015
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  • Good Attention Score compared to outputs of the same age (71st percentile)

Mentioned by

2 tweeters
1 peer review site
1 Facebook page
1 Google+ user


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Readers on

14 Mendeley
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Spatially transformed fluorescence image data for ERK-MAPK and selected proteins within human epidermis
Published in
Giga Science, December 2015
DOI 10.1186/s13742-015-0102-5
Pubmed ID

Joseph Cursons, Catherine E. Angel, Daniel G. Hurley, Cristin G. Print, P. Rod Dunbar, Marc D. Jacobs, Edmund J. Crampin


Phosphoprotein signalling pathways have been intensively studied in vitro, yet their role in regulating tissue homeostasis is not fully understood. In the skin, interfollicular keratinocytes differentiate over approximately 2 weeks as they traverse the epidermis. The extracellular signal-regulated kinase (ERK) branch of the mitogen-activated protein kinase (MAPK) pathway has been implicated in this process. Therefore, we examined ERK-MAPK activity within human epidermal keratinocytes in situ. We used confocal microscopy and immunofluorescence labelling to measure the relative abundances of Raf-1, MEK1/2 and ERK1/2, and their phosphorylated (active) forms within three human skin samples. Additionally, we measured the abundance of selected proteins thought to modulate ERK-MAPK activity, including calmodulin, β1 integrin and stratifin (14-3-3σ); and of transcription factors known to act as effectors of ERK1/2, including the AP-1 components Jun-B, Fra2 and c-Fos. Imaging was performed with sufficient resolution to identify the plasma membrane, cytoplasm and nucleus as distinct domains within cells across the epidermis. The image field of view was also sufficiently large to capture the entire epidermis in cross-section, and thus the full range of keratinocyte differentiation in a single observation. Image processing methods were developed to quantify image data for mathematical and statistical analysis. Here, we provide raw image data and processed outputs. These data indicate coordinated changes in ERK-MAPK signalling activity throughout the depth of the epidermis, with changes in relative phosphorylation-mediated signalling activity occurring along the gradient of cellular differentiation. We believe these data provide unique information about intracellular signalling as they are obtained from a homeostatic human tissue, and they might be useful for investigating intercellular heterogeneity.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 57%
Student > Bachelor 3 21%
Professor 1 7%
Student > Doctoral Student 1 7%
Student > Postgraduate 1 7%
Other 0 0%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 36%
Biochemistry, Genetics and Molecular Biology 4 29%
Medicine and Dentistry 2 14%
Materials Science 1 7%
Mathematics 1 7%
Other 0 0%
Unknown 1 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 January 2016.
All research outputs
of 12,012,688 outputs
Outputs from Giga Science
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Outputs of similar age
of 304,663 outputs
Outputs of similar age from Giga Science
of 23 outputs
Altmetric has tracked 12,012,688 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 457 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 23.4. This one is in the 10th percentile – i.e., 10% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 304,663 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one is in the 4th percentile – i.e., 4% of its contemporaries scored the same or lower than it.