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Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates

Overview of attention for article published in BMC Genomics, September 2018
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (79th percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

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Title
Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates
Published in
BMC Genomics, September 2018
DOI 10.1186/s12864-018-5065-3
Pubmed ID
Authors

Camino Trobajo-Sanmartín, Guillermo Ezpeleta, Célia Pais, Elena Eraso, Guillermo Quindós

Abstract

Analysis of polymorphic microsatellite markers (STR) is a helpful genotyping technique to differentiate Candida parapsilosis sensu stricto isolates. The aim of this study is to develop and perform an initial validation of an alternative protocol for the reliable and accurate microsatellite genotyping of C. parapsilosis sensu stricto isolates using high-throughput multiplex PCR. To achieve this, the results obtained using the new protocol were compared to the ones obtained using a previously described reference method. To that end, diagnostic accuracy, informativeness and discrimination parameters were estimated. Our results showed good concordance between both methods (Kappa index: 0.920), leading to a high sensitivity (1; CI(95%) (0.991-1)) and specificity (1; CI(95%) (0.772-1)) after the validation of the new protocol. Moreover, the electropherograms profiles obtained with the new PCR scheme showed a high signal to noise ratio (SNR). The new multiplex protocol is valuable for the differentiation of C. parapsilosis sensu stricto, with direct clinical applications. Besides, the new protocol represents a shortening the hands-on time, reducing the sample manipulation (dismissing the possibility of cross-contamination), maintaining the quality of the results (when compared to the ones obtained with the reference method), and helping to the standardization and simplification of the genotyping scheme.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 17%
Researcher 5 17%
Student > Ph. D. Student 5 17%
Other 2 7%
Student > Postgraduate 2 7%
Other 3 10%
Unknown 7 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 17%
Medicine and Dentistry 4 14%
Biochemistry, Genetics and Molecular Biology 3 10%
Immunology and Microbiology 2 7%
Materials Science 2 7%
Other 4 14%
Unknown 9 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 July 2020.
All research outputs
#3,330,183
of 23,105,443 outputs
Outputs from BMC Genomics
#1,330
of 10,709 outputs
Outputs of similar age
#70,527
of 342,007 outputs
Outputs of similar age from BMC Genomics
#31
of 182 outputs
Altmetric has tracked 23,105,443 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,709 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 342,007 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 182 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.