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An industrial scale process for the enzymatic removal of steryl glucosides from biodiesel

Overview of attention for article published in Biotechnology for Biofuels and Bioproducts, December 2015
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  • Good Attention Score compared to outputs of the same age (74th percentile)
  • Good Attention Score compared to outputs of the same age and source (68th percentile)

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Title
An industrial scale process for the enzymatic removal of steryl glucosides from biodiesel
Published in
Biotechnology for Biofuels and Bioproducts, December 2015
DOI 10.1186/s13068-015-0405-x
Pubmed ID
Authors

Salvador Peiru, Andres Aguirre, Florencia Eberhardt, Mauricio Braia, Rodolfo Cabrera, Hugo G. Menzella

Abstract

Biodiesels produced from transesterification of vegetable oils have a major quality problem due to the presence of precipitates, which need to be removed to avoid clogging of filters and engine failures. These precipitates have been reported to be mostly composed of steryl glucosides (SGs), but so far industrial cost-effective methods to remove these compounds are not available. Here we describe a novel method for the efficient removal of SGs from biodiesel, based on the hydrolytic activity of a thermostable β-glycosidase obtained from Thermococcus litoralis. A steryl glucosidase (SGase) enzyme from T. litoralis was produced and purified from Escherichia coli cultures expressing a synthetic gene, and used to treat soybean-derived biodiesel. Several optimization steps allowed for the selection of optimal reaction conditions to finally provide a simple and efficient process for the removal of SGs from crude biodiesel. The resulting biodiesel displayed filterability properties similar to distilled biodiesel according to the total contamination (TC), the cold soak filtration test (CSFT), filter blocking tendency (FBT), and cold soak filter blocking tendency (CSFBT) tests. The process was successfully scaled up to a 20 ton reactor, confirming its adaptability to industrial settings. The results presented in this work provide a novel path for the removal of steryl glucosides from biodiesel using a cost-effective, environmentally friendly and scalable enzymatic process, contributing to the adoption of this renewable fuel.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 2%
Unknown 44 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 12 27%
Student > Master 6 13%
Researcher 5 11%
Professor 4 9%
Student > Ph. D. Student 3 7%
Other 3 7%
Unknown 12 27%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 18%
Chemistry 8 18%
Biochemistry, Genetics and Molecular Biology 6 13%
Engineering 4 9%
Chemical Engineering 3 7%
Other 5 11%
Unknown 11 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 March 2016.
All research outputs
#7,047,742
of 25,374,647 outputs
Outputs from Biotechnology for Biofuels and Bioproducts
#455
of 1,578 outputs
Outputs of similar age
#101,017
of 396,020 outputs
Outputs of similar age from Biotechnology for Biofuels and Bioproducts
#16
of 54 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 1,578 research outputs from this source. They receive a mean Attention Score of 4.9. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 396,020 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 54 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.