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Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions

Overview of attention for article published in Biotechnology for Biofuels, January 2015
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (64th percentile)
  • Good Attention Score compared to outputs of the same age and source (79th percentile)

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3 tweeters
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1 Facebook page

Citations

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16 Dimensions

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38 Mendeley
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Title
Preservation of microbial communities enriched on lignocellulose under thermophilic and high-solid conditions
Published in
Biotechnology for Biofuels, January 2015
DOI 10.1186/s13068-015-0392-y
Pubmed ID
Authors

Yu, Chaowei, Reddy, Amitha P, Simmons, Christopher W, Simmons, Blake A, Singer, Steven W, VanderGheynst, Jean S

Abstract

Microbial communities enriched from diverse environments have shown considerable promise for the targeted discovery of microorganisms and enzymes for bioconversion of lignocellulose to liquid fuels. While preservation of microbial communities is important for commercialization and research, few studies have examined storage conditions ideal for preservation. The goal of this study was to evaluate the impact of preservation method on composition of microbial communities enriched on switchgrass before and after storage. The enrichments were completed in a high-solid and aerobic environment at 55 °C. Community composition was examined for each enrichment to determine when a stable community was achieved. Preservation methods included cryopreservation with the cryoprotective agents DMSO and glycerol, and cryopreservation without cryoprotective agents. Revived communities were examined for their ability to decompose switchgrass under high-solid and thermophilic conditions. High-throughput 16S rRNA gene sequencing of DNA extracted from enrichment samples showed that the majority of the shift in composition of the switchgrass-degrading community occurred during the initial three 2-week enrichments. Shifts in community structure upon storage occurred in all cryopreserved samples. Storage in liquid nitrogen in the absence of cryoprotectant resulted in variable preservation of dominant microorganisms in enriched samples. Cryopreservation with either DMSO or glycerol provided consistent and equivalent preservation of dominant organisms. A stable switchgrass-degrading microbial community was achieved after three 2-week enrichments. Dominant microorganisms were preserved equally well with DMSO and glycerol. DMSO-preserved communities required more incubation time upon revival to achieve pre-storage activity levels during high-solid thermophilic cultivation on switchgrass. Despite shifts in the community with storage, the samples were active upon revival under thermophilic and high-solid conditions. The results suggest that the presence of microorganisms may be more important than their relative abundance in retaining an active microbial community.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 38 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 42%
Researcher 7 18%
Student > Bachelor 4 11%
Unspecified 3 8%
Student > Master 3 8%
Other 5 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 53%
Unspecified 5 13%
Biochemistry, Genetics and Molecular Biology 4 11%
Environmental Science 3 8%
Chemical Engineering 1 3%
Other 5 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2016.
All research outputs
#3,936,063
of 9,271,416 outputs
Outputs from Biotechnology for Biofuels
#265
of 768 outputs
Outputs of similar age
#114,397
of 324,392 outputs
Outputs of similar age from Biotechnology for Biofuels
#19
of 94 outputs
Altmetric has tracked 9,271,416 research outputs across all sources so far. This one has received more attention than most of these and is in the 57th percentile.
So far Altmetric has tracked 768 research outputs from this source. They receive a mean Attention Score of 4.1. This one has gotten more attention than average, scoring higher than 65% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 324,392 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 94 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 79% of its contemporaries.