Title |
Simultaneous Multi-plane Imaging of Neural Circuits
|
---|---|
Published in |
Neuron, January 2016
|
DOI | 10.1016/j.neuron.2015.12.012 |
Pubmed ID | |
Authors |
Weijian Yang, Jae-eun Kang Miller, Luis Carrillo-Reid, Eftychios Pnevmatikakis, Liam Paninski, Rafael Yuste, Darcy S. Peterka |
Abstract |
Recording the activity of large populations of neurons is an important step toward understanding the emergent function of neural circuits. Here we present a simple holographic method to simultaneously perform two-photon calcium imaging of neuronal populations across multiple areas and layers of mouse cortex in vivo. We use prior knowledge of neuronal locations, activity sparsity, and a constrained nonnegative matrix factorization algorithm to extract signals from neurons imaged simultaneously and located in different focal planes or fields of view. Our laser multiplexing approach is simple and fast, and could be used as a general method to image the activity of neural circuits in three dimensions across multiple areas in the brain. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 11 | 28% |
Canada | 2 | 5% |
Japan | 2 | 5% |
Germany | 2 | 5% |
Norway | 1 | 3% |
Australia | 1 | 3% |
United Kingdom | 1 | 3% |
France | 1 | 3% |
Netherlands | 1 | 3% |
Other | 2 | 5% |
Unknown | 16 | 40% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 33 | 83% |
Scientists | 6 | 15% |
Science communicators (journalists, bloggers, editors) | 1 | 3% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 15 | 3% |
Germany | 4 | <1% |
France | 3 | <1% |
Switzerland | 1 | <1% |
Portugal | 1 | <1% |
United Kingdom | 1 | <1% |
China | 1 | <1% |
Netherlands | 1 | <1% |
Spain | 1 | <1% |
Other | 3 | <1% |
Unknown | 561 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 191 | 32% |
Researcher | 138 | 23% |
Student > Bachelor | 36 | 6% |
Student > Master | 29 | 5% |
Student > Postgraduate | 28 | 5% |
Other | 101 | 17% |
Unknown | 69 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 175 | 30% |
Agricultural and Biological Sciences | 149 | 25% |
Engineering | 74 | 13% |
Physics and Astronomy | 56 | 9% |
Biochemistry, Genetics and Molecular Biology | 15 | 3% |
Other | 51 | 9% |
Unknown | 72 | 12% |