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Sensitization by subcutaneous route is superior to intraperitoneal route in induction of asthma by house dust mite in a murine mode

Overview of attention for article published in Einstein (São Paulo), December 2015
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Title
Sensitization by subcutaneous route is superior to intraperitoneal route in induction of asthma by house dust mite in a murine mode
Published in
Einstein (São Paulo), December 2015
DOI 10.1590/s1679-45082015ao3389
Pubmed ID
Authors

Marcelo Vivolo Aun, Beatriz Mangueira Saraiva-Romanholo, Francine Maria de Almeida, Thayse Regina Brüggemann, Jorge Kalil, Milton de Arruda Martins, Fernanda Magalhães Arantes-Costa, Pedro Giavina-Bianchi

Abstract

Objective To develop a new experimental model of chronic allergic pulmonary disease induced by house dust mite, with marked production of specific immunoglobulin E (IgE), eosinophilic inflammatory infiltrate in the airways and remodeling, comparing two different routes of sensitization. Methods The protocol lasted 30 days. BALB/c mice were divided into six groups and were sensitized subcutaneously or intraperitoneally with saline (negative control), Dermatophagoides pteronyssinus (Der p) 50 or 500mcg in three injections. Subsequently they underwent intranasal challenge with Der p or saline for 7 days and were sacrificed 24 hours after the last challenge. We evaluated the titration of specific IgE anti-Der p, eosinophilic density in peribronchovascular space and airway remodeling. Results Both animals sensitized intraperitoneally and subcutaneously produced specific IgE anti-Der p. Peribronchovascular eosinophilia increased only in mice receiving lower doses of Der p. However, only the group sensitized with Der p 50mcg through subcutaneously route showed significant airway remodeling. Conclusion In this murine model of asthma, both pathways of sensitization led to the production of specific IgE and eosinophilia in the airways. However, only the subcutaneously route was able to induce remodeling. Furthermore, lower doses of Der p used in sensitization were better than higher ones, suggesting immune tolerance. Further studies are required to evaluate the efficacy of this model in the development of bronchial hyperresponsiveness, but it can already be replicated in experiments to create new therapeutic drugs or immunotherapeutic strategies.

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Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Other 5 18%
Researcher 4 14%
Student > Bachelor 3 11%
Student > Master 3 11%
Professor 1 4%
Other 2 7%
Unknown 10 36%
Readers by discipline Count As %
Medicine and Dentistry 6 21%
Agricultural and Biological Sciences 5 18%
Biochemistry, Genetics and Molecular Biology 3 11%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Immunology and Microbiology 1 4%
Other 3 11%
Unknown 9 32%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 August 2016.
All research outputs
#9,987,455
of 12,477,729 outputs
Outputs from Einstein (São Paulo)
#77
of 135 outputs
Outputs of similar age
#239,077
of 349,385 outputs
Outputs of similar age from Einstein (São Paulo)
#7
of 9 outputs
Altmetric has tracked 12,477,729 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 135 research outputs from this source. They receive a mean Attention Score of 3.1. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
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