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Natural transovarial transmission of dengue virus 4 in Aedes aegypti from Cuiabá, State of Mato Grosso, Brazil

Overview of attention for article published in Revista da Sociedade Brasileira de Medicina Tropical, February 2015
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Title
Natural transovarial transmission of dengue virus 4 in Aedes aegypti from Cuiabá, State of Mato Grosso, Brazil
Published in
Revista da Sociedade Brasileira de Medicina Tropical, February 2015
DOI 10.1590/0037-8682-0264-2014
Pubmed ID
Authors

Lucinéia Claudia de Toni Aquino da Cruz, Otacília Pereira Serra, Fábio Alexandre Leal-Santos, Ana Lucia Maria Ribeiro, Renata Dezengrini Slhessarenko, Marina Atanaka dos Santos

Abstract

Dengue is the most prevalent arboviral disease in tropical areas. In Mato Grosso, outbreaks are reported every year, but studies on dengue in this state are scarce. Natural transovarial infection of Aedes aegypti by a flavivirus was investigated in the Jardim Industriário neighborhood of Cuiabá, Mato Grosso. Eggs were collected with ovitraps during the dry, intermediate, and rainy seasons of 2012. After the eggs hatched and the larvae developed to adulthood, mosquitoes (n = 758) were identified and allocated to pools of 1-10 specimens according to the collection location, sex, and climatic period. After RNA extraction, multiplex semi-nested RT-PCR was performed to detect the four dengue virus (DENV) serotypes, yellow fever virus, West Nile virus and Saint Louis encephalitis virus. DENV-4 was the only flavivirus detected, and it was found in 8/50 pools (16.0%). Three of the positive pools contained females, and five contained males. Their nucleotide sequences presented 96-100% similarity with DENV-4 genotype II strains from Manaus, Amazonas. The minimum infection rate was 10.5 per 1000 specimens, and the maximum likelihood estimator of the infection rate was 11.6 (95% confidence interval: 4.8; 23.3). This study provides the first evidence of natural transovarial infection by DENV-4 in Ae. Aegypti in Mato Grosso, suggesting that this type of infection might serve as a mechanism of virus maintenance during interepidemic periods in Cuiabá, a city where dengue epidemics are reported every year. These results emphasize the need for efficient vector population control measures to prevent arbovirus outbreaks in the state.

Mendeley readers

The data shown below were compiled from readership statistics for 124 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 3 2%
United Kingdom 1 <1%
Unknown 120 97%

Demographic breakdown

Readers by professional status Count As %
Student > Master 27 22%
Student > Bachelor 22 18%
Researcher 21 17%
Student > Ph. D. Student 13 10%
Student > Doctoral Student 10 8%
Other 19 15%
Unknown 12 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 25%
Medicine and Dentistry 23 19%
Biochemistry, Genetics and Molecular Biology 19 15%
Immunology and Microbiology 7 6%
Environmental Science 7 6%
Other 22 18%
Unknown 15 12%