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A new improved protocol for in vitro intratubular dentinal bacterial contamination for antimicrobial endodontic tests: standardization and validation by confocal laser scanning microscopy

Overview of attention for article published in Journal of Applied Oral Science, July 2015
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Title
A new improved protocol for in vitro intratubular dentinal bacterial contamination for antimicrobial endodontic tests: standardization and validation by confocal laser scanning microscopy
Published in
Journal of Applied Oral Science, July 2015
DOI 10.1590/1678-775720140261
Pubmed ID
Authors

ANDRADE, Flaviana Bombarda de, ARIAS, Marcela Paola Castro, MALIZA, Amanda Garcia Alves, DUARTE, Marco Antonio Hungaro, GRAEFF, Márcia Sirlene Zardin, AMOROSO-SILVA, Pablo Andrés, MIDENA, Raquel Zanin, MORAES, Ivaldo Gomes de, Andrade, Flaviana Bombarda de, Arias, Marcela Paola Castro, Maliza, Amanda Garcia Alves, Duarte, Marco Antonio Hungaro, Graeff, Márcia Sirlene Zardin, Amoroso-Silva, Pablo Andrés, Midena, Raquel Zanin, Moraes, Ivaldo Gomes de

Abstract

Objectives To compare three methods of intratubular contamination that simulate endodontic infections using confocal laser scanning microscopy (CLSM). Material and Methods Two pre-existing models of dentinal contamination were used to induce intratubular infection (groups A and B). These methods were modified in an attempt to improve the model (group C). Among the modifications it may be included: specimen contamination for five days, ultrasonic bath with BHI broth after specimen sterilization, use of E. faecalis during the exponential growth phase, greater concentration of inoculum, and two cycles of centrifugation on alternate days with changes of culture media. All specimens were longitudinally sectioned and stained with of LIVE/DEAD® for 20 min. Specimens were assessed using CLSM, which provided images of the depth of viable bacterial proliferation inside the dentinal tubules. Additionally, three examiners used scores to classify the CLSM images according to the following parameters: homogeneity, density, and depth of the bacterial contamination inside the dentinal tubules. Kruskal-Wallis and Dunn's tests were used to evaluate the live and dead cells rates, and the scores obtained. Results The contamination scores revealed higher contamination levels in group C when compared with groups A and B (p<0.05). No differences were observed between group A and B (p>0.05). The volume of live cells in group C was higher than in groups A and B (p<0.05). Conclusion The new protocol for intratubular infection resulted in high and uniform patterns of bacterial contamination and higher cell viability in all specimens when compared with the current methods.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Egypt 1 2%
Unknown 65 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 18%
Student > Ph. D. Student 9 14%
Student > Bachelor 7 11%
Student > Doctoral Student 5 8%
Student > Postgraduate 5 8%
Other 9 14%
Unknown 19 29%
Readers by discipline Count As %
Medicine and Dentistry 36 55%
Agricultural and Biological Sciences 3 5%
Biochemistry, Genetics and Molecular Biology 2 3%
Nursing and Health Professions 1 2%
Immunology and Microbiology 1 2%
Other 1 2%
Unknown 22 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 July 2016.
All research outputs
#18,436,183
of 22,840,638 outputs
Outputs from Journal of Applied Oral Science
#274
of 499 outputs
Outputs of similar age
#189,769
of 264,109 outputs
Outputs of similar age from Journal of Applied Oral Science
#6
of 8 outputs
Altmetric has tracked 22,840,638 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 499 research outputs from this source. They receive a mean Attention Score of 2.6. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
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