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Toll-Like Receptors

Overview of attention for book
Toll-Like Receptors
Humana Press, New York, NY

Table of Contents

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    Book Overview
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    Chapter 1 Toll-Like Receptors: Ligands, Cell-Based Models, and Readouts for Receptor Action
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    Chapter 2 Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures.
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    Chapter 3 Toll-Like Receptor Interactions Measured by Microscopic and Flow Cytometric FRET
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    Chapter 4 Using Confocal Microscopy to Investigate Intracellular Trafficking of Toll-Like Receptors
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    Chapter 5 Assessing the Inhibitory Activity of Oligonucleotides on TLR7 Sensing.
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    Chapter 6 Methods for Delivering DNA to Intracellular Receptors
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    Chapter 7 Detection of Interaction Between Toll-Like Receptors and Other Transmembrane Proteins by Co-immunoprecipitation Assay
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    Chapter 8 Flow Cytometry-Based Bead-Binding Assay for Measuring Receptor Ligand Specificity
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    Chapter 9 Measuring Monomer-to-Filament Transition of MAVS as an In Vitro Activity Assay for RIG-I-Like Receptors
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    Chapter 10 Co-transcriptomic Analysis by RNA Sequencing to Simultaneously Measure Regulated Gene Expression in Host and Bacterial Pathogen
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    Chapter 11 Simple Methods to Investigate MicroRNA Induction in Response to Toll-Like Receptors.
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    Chapter 12 Determining the Function of Long Noncoding RNA in Innate Immunity.
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    Chapter 13 Analysis of Post-transcriptional Gene Regulation of Nod-Like Receptors via the 3'UTR.
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    Chapter 14 TLR Function in Murine CD4+ T Lymphocytes and Their Role in Inflammation
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    Chapter 15 Analysis by Flow Cytometry of B-Cell Activation and Antibody Responses Induced by Toll-Like Receptors.
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    Chapter 16 Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells.
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    Chapter 17 Analysis of TLR-Induced Metabolic Changes in Dendritic Cells Using the Seahorse XF(e)96 Extracellular Flux Analyzer.
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    Chapter 18 Toll-Like Receptor Signalling and the Control of Intestinal Barrier Function
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    Chapter 19 Understanding the Role of Cellular Molecular Clocks in Controlling the Innate Immune Response.
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    Chapter 20 Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis
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    Chapter 21 Allergens and Activation of the Toll-Like Receptor Response.
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    Chapter 22 Investigating the Role of Toll-Like Receptors in Models of Arthritis
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    Chapter 23 Delineating the Role of Toll-Like Receptors in the Neuro-inflammation Model EAE.
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    Chapter 24 The Use of MiRNA Antagonists in the Alleviation of Inflammatory Disorders.
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    Chapter 25 Investigating the Role of Toll-Like Receptors in Mouse Models of Gastric Cancer
Attention for Chapter 7: Detection of Interaction Between Toll-Like Receptors and Other Transmembrane Proteins by Co-immunoprecipitation Assay
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Chapter title
Detection of Interaction Between Toll-Like Receptors and Other Transmembrane Proteins by Co-immunoprecipitation Assay
Chapter number 7
Book title
Toll-Like Receptors
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3335-8_7
Pubmed ID
Book ISBNs
978-1-4939-3333-4, 978-1-4939-3335-8
Authors

Yu-Ran Lee, Wondae Kang, You-Me Kim

Abstract

Toll-like receptors are type I membrane proteins and bind other membrane proteins often via a specific interaction between transmembrane domains. The co-immunoprecipitation assay is a widely used biochemical technique for assessing interactions among proteins in cell lysates or tissue extracts. By isolating a native protein complex with a specific antibody against a protein of interest, followed by western blotting with an antibody for a binding partner, the co-immunoprecipitation assay can be used to confirm a putative interaction between two proteins. The co-immunoprecipitation assay can also be combined with a proteomics approach such as protein mass spectrometry to build an interactome of a target protein. Despite its usefulness and popularity to probe protein interactions within complex biological samples, the co-immunoprecipitation assay of membrane proteins is rather tricky, often resulting in false data. Here, we describe a co-immunoprecipitation method for analyzing interactions between toll-like receptors and other membrane proteins, using the interaction between TLR9 and UNC93B1 as an example. Especially, we describe an optimal cell lysis and sample preparation method to preserve protein interactions mediated by transmembrane domains.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 2 13%
Professor 2 13%
Researcher 2 13%
Student > Bachelor 1 7%
Unspecified 1 7%
Other 2 13%
Unknown 5 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 40%
Medicine and Dentistry 2 13%
Chemistry 1 7%
Unspecified 1 7%
Unknown 5 33%