Attention for Chapter 10:
Co-transcriptomic Analysis by RNA Sequencing to Simultaneously Measure Regulated Gene Expression in Host and Bacterial Pathogen
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Ravasi, Timothy, Mavromatis, Charalampos, Bokil, Nilesh J., Schembri, Mark A., Sweet, Matthew J., Timothy Ravasi, Charalampos (Harris) Mavromatis, Nilesh J. Bokil, Mark A. Schembri, Matthew J. Sweet
Abstract
Intramacrophage pathogens subvert antimicrobial defence pathways using various mechanisms, including the targeting of host TLR-mediated transcriptional responses. Conversely, TLR-inducible host defence mechanisms subject intramacrophage pathogens to stress, thus altering pathogen gene expression programs. Important biological insights can thus be gained through the analysis of gene expression changes in both the host and the pathogen during an infection. Traditionally, research methods have involved the use of qPCR, microarrays and/or RNA sequencing to identify transcriptional changes in either the host or the pathogen. Here we describe the application of RNA sequencing using samples obtained from in vitro infection assays to simultaneously quantify both host and bacterial pathogen gene expression changes, as well as general approaches that can be undertaken to interpret the RNA sequencing data that is generated. These methods can be used to provide insights into host TLR-regulated transcriptional responses to microbial challenge, as well as pathogen subversion mechanisms against such responses.
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 January 2016.
All research outputs
#17,782,514
of 22,840,638 outputs
Outputs from Methods in molecular biology
#7,242
of 13,127 outputs
Outputs of similar age
#267,679
of 393,571 outputs
Outputs of similar age from Methods in molecular biology
#752
of 1,470 outputs
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So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
