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Characterization of Sarcoptes scabiei cofilin gene and assessment of recombinant cofilin protein as an antigen in indirect-ELISA for diagnosis

Overview of attention for article published in BMC Infectious Diseases, January 2016
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Title
Characterization of Sarcoptes scabiei cofilin gene and assessment of recombinant cofilin protein as an antigen in indirect-ELISA for diagnosis
Published in
BMC Infectious Diseases, January 2016
DOI 10.1186/s12879-016-1353-1
Pubmed ID
Authors

Yu Zheng, Ran He, Manli He, Xiaobin Gu, Tao Wang, Weimin Lai, Xuerong Peng, Guangyou Yang

Abstract

Scabies impairs the health of humans and animals and causes heavy economic losses. Traditional diagnostic methods for scabies are inefficient and ineffective, and so far there is no commercial immunodiagnostic or molecular based test for scabies. Here, we used recombinant Sarcoptes scabiei cofilin protein as an antigen to establish indirect ELISA. S. scabiei cofilin is highly homologous to Dermatophagoides farinae Der f 31 allergen (90 % identity). The S. scabiei cofilin gene was cloned and expressed in Escherichia coli to obtain recombinant protein. Western blotting and fluorescence immunohistochemistry were carried out, and we established an indirect ELISA method and detected 33 serum samples from scabies infected rabbits and 30 serum samples from naïve rabbits. Western blotting demonstrated that S. scabiei cofilin possessed good immunogenicity and fluorescence immunohistochemistry showed the S. scabiei cofilin is widespread in the splanchnic area of mites. In ELISA, a cut-off value of 0.188 was determined to judge experimental positive and negative serum values. Specificity and sensitivity of the ELISA were 87.9 and 83.33 %, respectively. Recombinant S. scabiei cofilin showed potential value as a diagnostic antigen. The ELISA method established could be used in clinical diagnosis and provide experimental information in minimal or asymptomatic infection.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 13%
Student > Bachelor 2 8%
Researcher 2 8%
Student > Ph. D. Student 2 8%
Other 1 4%
Other 2 8%
Unknown 12 50%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 13%
Biochemistry, Genetics and Molecular Biology 3 13%
Medicine and Dentistry 3 13%
Veterinary Science and Veterinary Medicine 1 4%
Materials Science 1 4%
Other 0 0%
Unknown 13 54%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 January 2016.
All research outputs
#20,699,786
of 23,299,593 outputs
Outputs from BMC Infectious Diseases
#6,591
of 7,803 outputs
Outputs of similar age
#334,617
of 397,577 outputs
Outputs of similar age from BMC Infectious Diseases
#82
of 95 outputs
Altmetric has tracked 23,299,593 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
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We're also able to compare this research output to 95 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.