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Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential

Overview of attention for article published in Blood, April 2009
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (91st percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

Mentioned by

blogs
1 blog
patent
5 patents
wikipedia
1 Wikipedia page
f1000
1 research highlight platform

Citations

dimensions_citation
282 Dimensions

Readers on

mendeley
278 Mendeley
citeulike
7 CiteULike
connotea
3 Connotea
Title
Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential
Published in
Blood, April 2009
DOI 10.1182/blood-2008-12-192054
Pubmed ID
Authors

David G. Kent, Michael R. Copley, Claudia Benz, Stefan Wöhrer, Brad J. Dykstra, Elaine Ma, John Cheyne, Yongjun Zhao, Michelle B. Bowie, Yun Zhao, Maura Gasparetto, Allen Delaney, Clayton Smith, Marco Marra, Connie J. Eaves

Abstract

Hematopoietic stem cells (HSCs) are generally defined by their dual properties of pluripotency and extensive self-renewal capacity. However, a lack of experimental clarity as to what constitutes extensive self-renewal capacity coupled with an absence of methods to prospectively isolate long-term repopulating cells with defined self-renewal activities has made it difficult to identify the essential components of the self-renewal machinery and investigate their regulation. We now show that cells capable of repopulating irradiated congenic hosts for 4 months and producing clones of cells that can be serially transplanted are selectively and highly enriched in the CD150(+) subset of the EPCR(+)CD48(-)CD45(+) fraction of mouse fetal liver and adult bone marrow cells. In contrast, cells that repopulate primary hosts for the same period but show more limited self-renewal activity are enriched in the CD150(-) subset. Comparative transcriptome analyses of these 2 subsets with each other and with HSCs whose self-renewal activity has been rapidly extinguished in vitro revealed 3 new genes (VWF, Rhob, Pld3) whose elevated expression is a consistent and selective feature of the long-term repopulating cells with durable self-renewal capacity. These findings establish the identity of a phenotypically and molecularly distinct class of pluripotent hematopoietic cells with lifelong self-renewal capacity.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 278 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 2 <1%
United Kingdom 2 <1%
United States 2 <1%
Czechia 1 <1%
Canada 1 <1%
Switzerland 1 <1%
Unknown 269 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 83 30%
Researcher 67 24%
Student > Master 29 10%
Student > Doctoral Student 14 5%
Other 10 4%
Other 31 11%
Unknown 44 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 103 37%
Biochemistry, Genetics and Molecular Biology 72 26%
Medicine and Dentistry 31 11%
Immunology and Microbiology 9 3%
Engineering 6 2%
Other 8 3%
Unknown 49 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 July 2021.
All research outputs
#2,706,018
of 25,374,647 outputs
Outputs from Blood
#3,056
of 33,239 outputs
Outputs of similar age
#8,743
of 106,890 outputs
Outputs of similar age from Blood
#19
of 206 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 33,239 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.6. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 106,890 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 206 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.