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Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential

Overview of attention for article published in Blood, June 2009
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (92nd percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

blogs
1 blog
patent
5 patents
wikipedia
1 Wikipedia page
f1000
1 research highlight platform

Citations

dimensions_citation
251 Dimensions

Readers on

mendeley
259 Mendeley
citeulike
7 CiteULike
connotea
3 Connotea
Title
Prospective isolation and molecular characterization of hematopoietic stem cells with durable self-renewal potential
Published in
Blood, June 2009
DOI 10.1182/blood-2008-12-192054
Pubmed ID
Authors

David G. Kent, Michael R. Copley, Claudia Benz, Stefan Wöhrer, Brad J. Dykstra, Elaine Ma, John Cheyne, Yongjun Zhao, Michelle B. Bowie, Yun Zhao, Maura Gasparetto, Allen Delaney, Clayton Smith, Marco Marra, Connie J. Eaves

Abstract

Hematopoietic stem cells (HSCs) are generally defined by their dual properties of pluripotency and extensive self-renewal capacity. However, a lack of experimental clarity as to what constitutes extensive self-renewal capacity coupled with an absence of methods to prospectively isolate long-term repopulating cells with defined self-renewal activities has made it difficult to identify the essential components of the self-renewal machinery and investigate their regulation. We now show that cells capable of repopulating irradiated congenic hosts for 4 months and producing clones of cells that can be serially transplanted are selectively and highly enriched in the CD150(+) subset of the EPCR(+)CD48(-)CD45(+) fraction of mouse fetal liver and adult bone marrow cells. In contrast, cells that repopulate primary hosts for the same period but show more limited self-renewal activity are enriched in the CD150(-) subset. Comparative transcriptome analyses of these 2 subsets with each other and with HSCs whose self-renewal activity has been rapidly extinguished in vitro revealed 3 new genes (VWF, Rhob, Pld3) whose elevated expression is a consistent and selective feature of the long-term repopulating cells with durable self-renewal capacity. These findings establish the identity of a phenotypically and molecularly distinct class of pluripotent hematopoietic cells with lifelong self-renewal capacity.

Mendeley readers

The data shown below were compiled from readership statistics for 259 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 2 <1%
United Kingdom 2 <1%
United States 2 <1%
Czechia 1 <1%
Canada 1 <1%
Switzerland 1 <1%
Unknown 250 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 80 31%
Researcher 67 26%
Student > Master 28 11%
Student > Doctoral Student 14 5%
Other 9 3%
Other 27 10%
Unknown 34 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 102 39%
Biochemistry, Genetics and Molecular Biology 66 25%
Medicine and Dentistry 31 12%
Immunology and Microbiology 9 3%
Engineering 6 2%
Other 9 3%
Unknown 36 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 July 2021.
All research outputs
#1,996,401
of 20,114,180 outputs
Outputs from Blood
#2,267
of 29,038 outputs
Outputs of similar age
#19,380
of 243,848 outputs
Outputs of similar age from Blood
#22
of 383 outputs
Altmetric has tracked 20,114,180 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 29,038 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 243,848 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 383 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.