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Agrobacterium Biology

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Cover of 'Agrobacterium Biology'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 80 Agrobacterium -Mediated Transformation in the Evolution of Plants
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    Chapter 81 One More Decade of Agrobacterium Taxonomy
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    Chapter 82 Beyond Agrobacterium-Mediated Transformation: Horizontal Gene Transfer from Bacteria to Eukaryotes
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    Chapter 83 Niche Construction and Exploitation by Agrobacterium: How to Survive and Face Competition in Soil and Plant Habitats
  6. Altmetric Badge
    Chapter 84 Small Noncoding RNAs in Agrobacterium tumefaciens
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    Chapter 85 The Ecology of Agrobacterium vitis and Management of Crown Gall Disease in Vineyards
  8. Altmetric Badge
    Chapter 90 Agrobacterium-Mediated Transformation of Yeast and Fungi
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    Chapter 92 Cell Wall Biogenesis During Elongation and Division in the Plant Pathogen Agrobacterium tumefaciens
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    Chapter 93 The Agrobacterium Phenotypic Plasticity ( Plast ) Genes
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    Chapter 94 The Agrobacterium VirB/VirD4 T4SS: Mechanism and Architecture Defined Through In Vivo Mutagenesis and Chimeric Systems
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    Chapter 96 Function and Regulation of Agrobacterium tumefaciens Cell Surface Structures that Promote Attachment
  13. Altmetric Badge
    Chapter 97 Advancing Agrobacterium-Based Crop Transformation and Genome Modification Technology for Agricultural Biotechnology
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    Chapter 98 The Mechanism of T-DNA Integration: Some Major Unresolved Questions
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    Chapter 99 The Agrobacterium Type VI Secretion System: A Contractile Nanomachine for Interbacterial Competition
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    Chapter 100 Exopolysaccharides of Agrobacterium tumefaciens
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    Chapter 101 Agrobacterium : A Genome-Editing Tool-Delivery System
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    Chapter 115 Transcriptome Profiling of Plant Genes in Response to Agrobacterium Tumefaciens-Mediated Transformation
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    Chapter 119 Coping with High Temperature: A Unique Regulation in A. tumefaciens
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    Chapter 131 Real-Time Trafficking of Agrobacterium Virulence Protein VirE2 Inside Host Cells
  21. Altmetric Badge
    Chapter 136 Correction to: Agrobacterium : A Genome-Editing Tool-Delivery System
Attention for Chapter 90: Agrobacterium-Mediated Transformation of Yeast and Fungi
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Chapter title
Agrobacterium-Mediated Transformation of Yeast and Fungi
Chapter number 90
Book title
Agrobacterium Biology
Published in
Current topics in microbiology and immunology, January 2018
DOI 10.1007/82_2018_90
Pubmed ID
Book ISBNs
978-3-03-003256-2, 978-3-03-003257-9
Authors

Paul J. J. Hooykaas, G. Paul H. van Heusden, Xiaolei Niu, M. Reza Roushan, Jalal Soltani, Xiaorong Zhang, Bert J. van der Zaal, Hooykaas, Paul J. J., van Heusden, G. Paul H., Niu, Xiaolei, Reza Roushan, M., Soltani, Jalal, Zhang, Xiaorong, van der Zaal, Bert J.

Abstract

Two decades ago, it was discovered that the well-known plant vector Agrobacterium tumefaciens can also transform yeasts and fungi when these microorganisms are co-cultivated on a solid substrate in the presence of a phenolic inducer such as acetosyringone. It is important that the medium has a low pH (5-6) and that the temperature is kept at room temperature (20-25 °C) during co-cultivation. Nowadays, Agrobacterium-mediated transformation (AMT) is the method of choice for the transformation of many fungal species; as the method is simple, the transformation efficiencies are much higher than with other methods, and AMT leads to single-copy integration much more frequently than do other methods. Integration of T-DNA in fungi occurs by non-homologous end-joining (NHEJ), but also targeted integration of the T-DNA by homologous recombination (HR) is possible. In contrast to AMT of plants, which relies on the assistance of a number of translocated virulence (effector) proteins, none of these (VirE2, VirE3, VirD5, VirF) are necessary for AMT of yeast or fungi. This is in line with the idea that some of these proteins help to overcome plant defense. Importantly, it also showed that VirE2 is not necessary for the transport of the T-strand into the nucleus. The yeast Saccharomyces cerevisiae is a fast-growing organism with a relatively simple genome with reduced genetic redundancy. This yeast species has therefore been used to unravel basic molecular processes in eukaryotic cells as well as to elucidate the function of virulence factors of pathogenic microorganisms acting in plants or animals. Translocation of Agrobacterium virulence proteins into yeast was recently visualized in real time by confocal microscopy. In addition, the yeast 2-hybrid system, one of many tools that have been developed for use in this yeast, was used to identify plant and yeast proteins interacting with the translocated Agrobacterium virulence proteins. Dedicated mutant libraries, containing for each gene a mutant with a precise deletion, have been used to unravel the mode of action of some of the Agrobacterium virulence proteins. Yeast deletion mutant collections were also helpful in identifying host factors promoting or inhibiting AMT, including factors involved in T-DNA integration. Thus, the homologous recombination (HR) factor Rad52 was found to be essential for targeted integration of T-DNA by HR in yeast. Proteins mediating double-strand break (DSB) repair by end-joining (Ku70, Ku80, Lig4) turned out to be essential for non-homologous integration. Inactivation of any one of the genes encoding these end-joining factors in other yeasts and fungi was employed to reduce or totally eliminate non-homologous integration and promote efficient targeted integration at the homologous locus by HR. In plants, however, their inactivation did not prevent non-homologous integration, indicating that T-DNA is captured by different DNA repair pathways in plants and fungi.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 49 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 16%
Student > Master 8 16%
Researcher 7 14%
Student > Ph. D. Student 5 10%
Student > Doctoral Student 3 6%
Other 4 8%
Unknown 14 29%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 33%
Biochemistry, Genetics and Molecular Biology 13 27%
Nursing and Health Professions 1 2%
Immunology and Microbiology 1 2%
Social Sciences 1 2%
Other 3 6%
Unknown 14 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 June 2018.
All research outputs
#18,640,437
of 23,092,602 outputs
Outputs from Current topics in microbiology and immunology
#531
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Outputs of similar age
#330,852
of 442,643 outputs
Outputs of similar age from Current topics in microbiology and immunology
#7
of 10 outputs
Altmetric has tracked 23,092,602 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 683 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.9. This one is in the 12th percentile – i.e., 12% of its peers scored the same or lower than it.
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