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A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine

Overview of attention for article published in Plant Methods, January 1970
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Citations

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Title
A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine
Published in
Plant Methods, January 1970
DOI 10.1186/1746-4811-6-5
Pubmed ID
Authors

Kotogány E, Dudits D, Horváth GV, Ayaydin F

Abstract

Progress in plant cell cycle research is highly dependent on reliable methods for detection of cells replicating DNA. Frequency of S-phase cells (cells in DNA synthesis phase) is a basic parameter in studies on the control of cell division cycle and the developmental events of plant cells. Here we extend the microscopy and flow cytometry applications of the recently developed EdU (5-ethynyl-2'-deoxyuridine)-based S-phase assay to various plant species and tissues. We demonstrate that the presented protocols insure the improved preservation of cell and tissue structure and allow significant reduction in assay duration. In comparison with the frequently used detection of bromodeoxyuridine (BrdU) and tritiated-thymidine incorporation, this new methodology offers several advantages as we discuss here.

Mendeley readers

The data shown below were compiled from readership statistics for 178 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 1%
Taiwan 1 <1%
Portugal 1 <1%
Australia 1 <1%
United Kingdom 1 <1%
Spain 1 <1%
Japan 1 <1%
India 1 <1%
Czechia 1 <1%
Other 2 1%
Unknown 166 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 58 33%
Student > Ph. D. Student 55 31%
Student > Bachelor 15 8%
Student > Master 14 8%
Professor > Associate Professor 8 4%
Other 28 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 135 76%
Biochemistry, Genetics and Molecular Biology 22 12%
Chemistry 6 3%
Medicine and Dentistry 4 2%
Unspecified 3 2%
Other 8 4%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 March 2010.
All research outputs
#2,015,273
of 3,631,967 outputs
Outputs from Plant Methods
#102
of 142 outputs
Outputs of similar age
#99,876
of 231,909 outputs
Outputs of similar age from Plant Methods
#2
of 6 outputs
Altmetric has tracked 3,631,967 research outputs across all sources so far. This one is in the 26th percentile – i.e., 26% of other outputs scored the same or lower than it.
So far Altmetric has tracked 142 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 231,909 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.