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A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine

Overview of attention for article published in Plant Methods, January 2010
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Citations

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Title
A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine
Published in
Plant Methods, January 2010
DOI 10.1186/1746-4811-6-5
Pubmed ID
Authors

Edit Kotogány, Dénes Dudits, Gábor V Horváth, Ferhan Ayaydin

Abstract

Progress in plant cell cycle research is highly dependent on reliable methods for detection of cells replicating DNA. Frequency of S-phase cells (cells in DNA synthesis phase) is a basic parameter in studies on the control of cell division cycle and the developmental events of plant cells. Here we extend the microscopy and flow cytometry applications of the recently developed EdU (5-ethynyl-2'-deoxyuridine)-based S-phase assay to various plant species and tissues. We demonstrate that the presented protocols insure the improved preservation of cell and tissue structure and allow significant reduction in assay duration. In comparison with the frequently used detection of bromodeoxyuridine (BrdU) and tritiated-thymidine incorporation, this new methodology offers several advantages as we discuss here.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 242 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 <1%
Australia 1 <1%
India 1 <1%
Canada 1 <1%
Portugal 1 <1%
Taiwan 1 <1%
Singapore 1 <1%
Japan 1 <1%
Spain 1 <1%
Other 0 0%
Unknown 232 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 68 28%
Student > Ph. D. Student 65 27%
Student > Master 21 9%
Student > Bachelor 19 8%
Professor > Associate Professor 10 4%
Other 29 12%
Unknown 30 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 153 63%
Biochemistry, Genetics and Molecular Biology 34 14%
Medicine and Dentistry 7 3%
Chemistry 7 3%
Computer Science 2 <1%
Other 10 4%
Unknown 29 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 March 2010.
All research outputs
#15,240,835
of 22,660,862 outputs
Outputs from Plant Methods
#820
of 1,070 outputs
Outputs of similar age
#134,179
of 164,423 outputs
Outputs of similar age from Plant Methods
#5
of 5 outputs
Altmetric has tracked 22,660,862 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,070 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 164,423 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one.