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Rbf Promotes Biofilm Formation by Staphylococcus aureus via Repression of icaR, a Negative Regulator of icaADBC

Overview of attention for article published in Journal of Bacteriology, October 2009
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Title
Rbf Promotes Biofilm Formation by Staphylococcus aureus via Repression of icaR, a Negative Regulator of icaADBC
Published in
Journal of Bacteriology, October 2009
DOI 10.1128/jb.00913-09
Pubmed ID
Authors

David Cue, Mei G. Lei, Thanh T. Luong, Lisa Kuechenmeister, Paul M. Dunman, Sinead O'Donnell, Sarah Rowe, James P. O'Gara, Chia Y. Lee

Abstract

We previously reported the identification of a gene, rbf, involved in the regulation of biofilm formation by Staphylococcus aureus 8325-4. In an effort to study the mechanism of regulation, microarrays were used to compare the transcription profiles of the wild-type strain with an rbf mutant and an rbf overexpression strain of the clinical isolate UAMS-1. Among the genes affected by rbf overexpression are those of the intercellular adhesion (ica) locus; however, expression of these genes was not affected by an rbf deletion in the chromosome. The icaADBC genes are responsible for production of poly-N-acetylglucosamine (PNAG), a major constituent of biofilm. The icaR gene encodes a negative regulator of icaADBC. In UAMS-1 carrying an Rbf-encoding plasmid, Rbf was found to repress icaR transcription with a concomitant increase in icaADBC expression and increased PNAG and biofilm production relative to isogenic strains lacking the plasmid. Sequencing of the rbf gene from UAMS-1 showed that there was a 2-bp insertion affecting the 50th codon of the rbf open reading frame, suggesting that rbf is a pseudogene in UAMS-1. This finding explains why deletion of rbf had no effect on biofilm formation in UAMS-1. To further characterize the Rbf regulation on biofilm we compared biofilm formation, icaA and icaR transcription, and PNAG production in 8325-4 and its isogenic rbf and icaR single mutants and an rbf icaR double mutant. Our results are consistent with a model wherein rbf represses synthesis of icaR, which in turn results in derepression of icaADBC and increased PNAG production. Furthermore, purified rbf did not bind to the icaR or icaA promoter region, suggesting that rbf controls expression of an unknown factor(s) that represses icaR. The role of rbf in controlling the S. aureus biofilm phenotype was further demonstrated in a clinical strain, MW2.

Mendeley readers

The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 1%
Spain 1 1%
Greece 1 1%
Unknown 68 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 21%
Researcher 13 18%
Student > Master 9 13%
Student > Bachelor 6 8%
Professor > Associate Professor 6 8%
Other 15 21%
Unknown 7 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 28 39%
Biochemistry, Genetics and Molecular Biology 13 18%
Immunology and Microbiology 10 14%
Medicine and Dentistry 4 6%
Chemistry 2 3%
Other 3 4%
Unknown 11 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 October 2009.
All research outputs
#9,196,132
of 14,612,563 outputs
Outputs from Journal of Bacteriology
#9,801
of 11,304 outputs
Outputs of similar age
#115,993
of 212,945 outputs
Outputs of similar age from Journal of Bacteriology
#38
of 63 outputs
Altmetric has tracked 14,612,563 research outputs across all sources so far. This one is in the 24th percentile – i.e., 24% of other outputs scored the same or lower than it.
So far Altmetric has tracked 11,304 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 212,945 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 63 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.