Chapter title |
Subcellular Targeting of Proteins Involved in Modification of Plant N- and O-Glycosylation
|
---|---|
Chapter number | 18 |
Book title |
Glyco-Engineering
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2760-9_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2759-3, 978-1-4939-2760-9
|
Authors |
Martina Dicker, Jennifer Schoberer, Ulrike Vavra, Richard Strasser |
Editors |
Alexandra Castilho |
Abstract |
Plants are attractive expression hosts for the production of recombinant glycoprotein therapeutics. The quality and efficiency of these biopharmaceuticals are very often influenced by the glycosylation profile. Consequently, approaches are needed that enable the production of recombinant glycoproteins with customized and homogenous N- and O-glycan structures. Here, we describe convenient tools that allow targeting and retention of glycan-modifying enzymes in the early secretory pathway of plants. These protocols can be used to fine-tune the subcellular localization of glycosyltransferases and glycosidases in plants and consequently to increase the homogeneity of glycosylation on recombinant glycoproteins. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 30% |
Researcher | 2 | 20% |
Student > Bachelor | 1 | 10% |
Student > Master | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Other | 0 | 0% |
Unknown | 2 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 60% |
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Unknown | 2 | 20% |