| Chapter title |
Glyco-Engineering
|
|---|---|
| Chapter number | 15 |
| Book title |
Glyco-Engineering
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2760-9_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2759-3, 978-1-4939-2760-9
|
| Authors |
Decker, Eva L, Wiedemann, Gertrud, Reski, Ralf, Eva L. Decker, Gertrud Wiedemann, Ralf Reski |
| Abstract |
One of the main challenges for the production of biopharmaceuticals in plant-based systems is the modulation of plant-specific glycosylation patterns towards a humanized form. Posttranslational modifications in plants are similar to those in humans, but several differences affect product quality and efficacy and can also cause immune responses in patients. In the moss Physcomitrella patens highly efficient gene targeting via homologous recombination enables glyco-engineering to obtain suitable platform lines for the production of recombinant proteins and biopharmaceuticals. Here we describe the methods which are effective for creating gene targeting constructs and transgenic moss lines as well as confirming successful homologous integration of the constructs and modification of target gene expression. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 4% |
| Unknown | 23 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 25% |
| Researcher | 4 | 17% |
| Student > Master | 4 | 17% |
| Student > Ph. D. Student | 3 | 13% |
| Professor | 1 | 4% |
| Other | 3 | 13% |
| Unknown | 3 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 46% |
| Agricultural and Biological Sciences | 10 | 42% |
| Unknown | 3 | 13% |