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Mendeley readers
| Chapter title |
Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries
|
|---|---|
| Chapter number | 10 |
| Book title |
Drosophila Oogenesis
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2851-4_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2850-7, 978-1-4939-2851-4
|
| Authors |
Zehra F. Nizami, Ji-Long Liu, Joseph G. Gall |
| Abstract |
Fluorescent in situ hybridization (FISH) is a technique for determining the cytological localization of RNA or DNA molecules. There are many approaches available for generating in situ hybridization probes and conducting the subsequent hybridization steps. Here, we describe a simple and reliable FISH method to label small RNAs (200-500 nucleotides in length) that are enriched in nuclear bodies in Drosophila melanogaster ovaries, such as Cajal bodies (CBs) and histone locus bodies (HLBs). This technique can also be applied to other Drosophila tissues, and to abundant mRNAs such as histone transcripts. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 33% |
| Researcher | 1 | 17% |
| Unknown | 3 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 33% |
| Agricultural and Biological Sciences | 1 | 17% |
| Chemistry | 1 | 17% |
| Unknown | 2 | 33% |