| Chapter title |
Patient-Specific Induced Pluripotent Stem Cell Models
|
|---|---|
| Chapter number | 267 |
| Book title |
Patient-Specific Induced Pluripotent Stem Cell Models
|
| Published in |
Methods in molecular biology, May 2015
|
| DOI | 10.1007/7651_2015_267 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3033-3, 978-1-4939-3034-0
|
| Authors |
Walter, Anna, Šarić, Tomo, Hescheler, Jürgen, Papadopoulos, Symeon, Anna Walter, Tomo Šarić, Jürgen Hescheler, Symeon Papadopoulos |
| Editors |
Andras Nagy, Kursad Turksen |
| Abstract |
The possibility to generate cardiomyocytes (CMs) from disease-specific induced pluripotent stem cells (iPSCs) is a powerful tool for the investigation of various cardiac diseases in vitro. The pathological course of various cardiac conditions, causatively heterogeneous, often converges into disturbed cellular Ca(2+) cycling. The gigantic Ca(2+) channel of the intracellular Ca(2+) store of CMs, the ryanodine receptor type 2 (RyR2), controls Ca(2+) release and therefore plays a crucial role in Ca(2+) cycling of CMs. In the present protocol we describe ways to measure and analyze global as well as local cellular Ca(2+) release events in CMs derived from a patient carrying a CPVT-causing RyR2 mutation. |
Mendeley readers
Geographical breakdown
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|---|---|---|
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| Unknown | 27 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
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| Student > Doctoral Student | 4 | 14% |
| Student > Master | 3 | 11% |
| Student > Ph. D. Student | 3 | 11% |
| Professor | 2 | 7% |
| Other | 4 | 14% |
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|---|---|---|
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| Neuroscience | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 10 | 36% |