Chapter title |
Modeling Axonal Phenotypes with Human Pluripotent Stem Cells.
|
---|---|
Chapter number | 167 |
Book title |
Patient-Specific Induced Pluripotent Stem Cell Models
|
Published in |
Methods in molecular biology, December 2014
|
DOI | 10.1007/7651_2014_167 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3033-3, 978-1-4939-3034-0
|
Authors |
Kyle R. Denton, Chong-Chong Xu, Xue-Jun Li |
Editors |
Andras Nagy, Kursad Turksen |
Abstract |
Impaired axonal development and degeneration are implicated in many debilitating disorders, such as hereditary spastic paraplegia (HSP), amyotrophic lateral sclerosis (ALS), and periphery neuropathy. Human pluripotent stem cells (hPSCs) have provided researchers with an excellent resource for modeling human neuropathologic processes including axonal defects in vitro. There are a number of steps that are crucial when developing an hPSC-based model of a human disease, including generating induced pluripotent stem cells (iPSCs), differentiating those cells to affected cell types, and identifying disease-relevant phenotypes. Here, we describe these steps in detail, focusing on the neurodegenerative disorder HSP. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 5 | 22% |
Student > Ph. D. Student | 4 | 17% |
Researcher | 3 | 13% |
Other | 2 | 9% |
Student > Postgraduate | 2 | 9% |
Other | 3 | 13% |
Unknown | 4 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 22% |
Medicine and Dentistry | 5 | 22% |
Neuroscience | 5 | 22% |
Social Sciences | 1 | 4% |
Nursing and Health Professions | 1 | 4% |
Other | 2 | 9% |
Unknown | 4 | 17% |