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Mendeley readers
Attention Score in Context
| Title |
A membrane protein preserves intrabacterial pH in intraphagosomal Mycobacterium tuberculosis
|
|---|---|
| Published in |
Nature Medicine, July 2008
|
| DOI | 10.1038/nm.1795 |
| Pubmed ID | |
| Authors |
Omar H Vandal, Lynda M Pierini, Dirk Schnappinger, Carl F Nathan, Sabine Ehrt |
| Abstract |
Acidification of the phagosome is considered to be a major mechanism used by macrophages against bacteria, including Mycobacterium tuberculosis (Mtb). Mtb blocks phagosome acidification, but interferon-gamma (IFN-gamma) restores acidification and confers antimycobacterial activity. Nonetheless, it remains unclear whether acid kills Mtb, whether the intrabacterial pH of any pathogen falls when it is in the phagosome and whether acid resistance is required for mycobacterial virulence. In vitro at pH 4.5, Mtb survived in a simple buffer and maintained intrabacterial pH. Therefore, Mtb resists phagolysosomal concentrations of acid. Mtb also maintained its intrabacterial pH and survived when phagocytosed by IFN-gamma-activated macrophages. We used transposon mutagenesis to identify genes responsible for Mtb's acid resistance. A strain disrupted in Rv3671c, a previously uncharacterized gene encoding a membrane-associated protein, was sensitive to acid and failed to maintain intrabacterial pH in acid in vitro and in activated macrophages. Growth of the mutant was also severely attenuated in mice. Thus, Mtb is able to resist acid, owing in large part to Rv3671c, and this resistance is essential for virulence. Disruption of Mtb's acid resistance and intrabacterial pH maintenance systems is an attractive target for chemotherapy. |
Mendeley readers
The data shown below were compiled from readership statistics for 300 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 2% |
| Germany | 4 | 1% |
| United Kingdom | 4 | 1% |
| Mexico | 2 | <1% |
| Israel | 1 | <1% |
| Switzerland | 1 | <1% |
| Norway | 1 | <1% |
| South Africa | 1 | <1% |
| Unknown | 281 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 83 | 28% |
| Researcher | 59 | 20% |
| Student > Master | 29 | 10% |
| Student > Bachelor | 28 | 9% |
| Student > Doctoral Student | 20 | 7% |
| Other | 45 | 15% |
| Unknown | 36 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 112 | 37% |
| Biochemistry, Genetics and Molecular Biology | 59 | 20% |
| Immunology and Microbiology | 34 | 11% |
| Medicine and Dentistry | 17 | 6% |
| Chemistry | 9 | 3% |
| Other | 22 | 7% |
| Unknown | 47 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 September 2022.
All research outputs
#2,520,383
of 23,317,888 outputs
Outputs from Nature Medicine
#3,857
of 8,610 outputs
Outputs of similar age
#7,033
of 82,646 outputs
Outputs of similar age from Nature Medicine
#17
of 47 outputs
Altmetric has tracked 23,317,888 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,610 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 100.1. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 82,646 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.