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Calpain

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Cover of 'Calpain'

Table of Contents

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    Book Overview
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    Chapter 1 Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin Using Anion-Exchange Chromatography
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    Chapter 2 Bacterial Expression and Purification of Calpains
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    Chapter 3 Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity
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    Chapter 4 FRET-Based Assays to Determine Calpain Activity
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    Chapter 5 In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer
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    Chapter 6 Determining Temporal and Spatial Expression of Calpains in Amphibians
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    Chapter 7 Immunohistochemical Localization of Calpains in the Amphibian Xenopus laevis
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    Chapter 8 Expression and Activity of Calpain A in Drosophila melanogaster
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    Chapter 9 Use of the β-Glucuronidase (GUS) Reporter System to Localize Promoter Activities of the Endogenous Plant Calpain DEFECTIVE KERNEL1 (DEK1)
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    Chapter 10 LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence
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    Chapter 11 CalCleaveMKL: a Tool for Calpain Cleavage Prediction
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    Chapter 12 Identification of Calpain-Activated Protein Functions
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    Chapter 13 Production and Purification of Recombinant Calpastatin
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    Chapter 14 Isolation of Endogenous Calpastatin
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    Chapter 15 Immunoblotting for Calpastatin Expression
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    Chapter 16 Experimental Manipulation of Calpain Activity In Vitro
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    Chapter 17 In Vivo Calpain Knockdown Using Delivery of siRNA
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    Chapter 18 In Vivo Expression of Mutant Calpains in the Eye Using Lentivirus
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    Chapter 19 Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians
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    Chapter 20 Genetic Models of Calpain Deficiency and Ectopic Expression
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Title
Calpain
Published by
Springer New York, January 2019
DOI 10.1007/978-1-4939-8988-1
ISBNs
978-1-4939-8987-4, 978-1-4939-8988-1
Editors

Jeannette S. Messer

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