↓ Skip to main content

TIMPs of parasitic helminths – a large-scale analysis of high-throughput sequence datasets

Overview of attention for article published in Parasites & Vectors, May 2013
Altmetric Badge

Citations

dimensions_citation
13 Dimensions

Readers on

mendeley
49 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
TIMPs of parasitic helminths – a large-scale analysis of high-throughput sequence datasets
Published in
Parasites & Vectors, May 2013
DOI 10.1186/1756-3305-6-156
Pubmed ID
Authors

Cinzia Cantacessi, Andreas Hofmann, Darren Pickering, Severine Navarro, Makedonka Mitreva, Alex Loukas

Abstract

Tissue inhibitors of metalloproteases (TIMPs) are a multifunctional family of proteins that orchestrate extracellular matrix turnover, tissue remodelling and other cellular processes. In parasitic helminths, such as hookworms, TIMPs have been proposed to play key roles in the host-parasite interplay, including invasion of and establishment in the vertebrate animal hosts. Currently, knowledge of helminth TIMPs is limited to a small number of studies on canine hookworms, whereas no information is available on the occurrence of TIMPs in other parasitic helminths causing neglected diseases. In the present study, we conducted a large-scale investigation of TIMP proteins of a range of neglected human parasites including the hookworm Necator americanus, the roundworm Ascaris suum, the liver flukes Clonorchis sinensis and Opisthorchis viverrini, as well as the schistosome blood flukes. This entailed mining available transcriptomic and/or genomic sequence datasets for the presence of homologues of known TIMPs, predicting secondary structures of defined protein sequences, systematic phylogenetic analyses and assessment of differential expression of genes encoding putative TIMPs in the developmental stages of A. suum, N. americanus and Schistosoma haematobium which infect the mammalian hosts. A total of 15 protein sequences with high homology to known eukaryotic TIMPs were predicted from the complement of sequence data available for parasitic helminths and subjected to in-depth bioinformatic analyses. Supported by the availability of gene manipulation technologies such as RNA interference and/or transgenesis, this work provides a basis for future functional explorations of helminth TIMPs and, in particular, of their role/s in fundamental biological pathways linked to long-term establishment in the vertebrate hosts, with a view towards the development of novel approaches for the control of neglected helminthiases.

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Indonesia 1 2%
India 1 2%
Uruguay 1 2%
United States 1 2%
Unknown 45 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 24%
Unspecified 8 16%
Researcher 7 14%
Student > Bachelor 6 12%
Student > Master 5 10%
Other 11 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 39%
Unspecified 9 18%
Immunology and Microbiology 7 14%
Biochemistry, Genetics and Molecular Biology 3 6%
Veterinary Science and Veterinary Medicine 2 4%
Other 9 18%