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Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method

Overview of attention for article published in BMC Biotechnology, January 2012
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Mentioned by

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2 tweeters

Citations

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35 Dimensions

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54 Mendeley
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Title
Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method
Published in
BMC Biotechnology, January 2012
DOI 10.1186/1472-6750-12-1
Pubmed ID
Authors

Eriko Kage-Nakadai, Hiroyuki Kobuna, Osamu Funatsu, Muneyoshi Otori, Keiko Gengyo-Ando, Sawako Yoshina, Sayaka Hori, Shohei Mitani

Abstract

Transgenic strains of Caenorhabditis elegans are typically generated by injecting DNA into the germline to form multi-copy extrachromosomal arrays. These transgenes are semi-stable and their expression is silenced in the germline. Mos1 transposon or microparticle bombardment methods have been developed to create single- or low-copy chromosomal integrated lines. Here we report an alternative method using ultraviolet trimethylpsoralen (UV/TMP) to generate single/low-copy gene integrations.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 7%
Unknown 50 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 16 30%
Student > Ph. D. Student 12 22%
Professor > Associate Professor 6 11%
Professor 5 9%
Student > Postgraduate 3 6%
Other 7 13%
Unknown 5 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 32 59%
Biochemistry, Genetics and Molecular Biology 10 19%
Neuroscience 2 4%
Physics and Astronomy 1 2%
Immunology and Microbiology 1 2%
Other 2 4%
Unknown 6 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 January 2012.
All research outputs
#7,141,790
of 12,373,386 outputs
Outputs from BMC Biotechnology
#442
of 695 outputs
Outputs of similar age
#103,457
of 223,324 outputs
Outputs of similar age from BMC Biotechnology
#21
of 33 outputs
Altmetric has tracked 12,373,386 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 695 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 223,324 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.