Title |
Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis)
|
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Published in |
BMC Biotechnology, September 2011
|
DOI | 10.1186/1472-6750-11-85 |
Pubmed ID | |
Authors |
Gustavo R Oliveira, Maria CM Silva, Wagner A Lucena, Erich YT Nakasu, Alexandre AP Firmino, Magda A Beneventi, Djair SL Souza, José E Gomes, José DA de Souza, Daniel J Rigden, Hudson B Ramos, Carlos R Soccol, Maria F Grossi-de-Sa |
Abstract |
The cotton boll weevil (Anthonomus grandis) is a serious insect-pest in the Americas, particularly in Brazil. The use of chemical or biological insect control is not effective against the cotton boll weevil because of its endophytic life style. Therefore, the use of biotechnological tools to produce insect-resistant transgenic plants represents an important strategy to reduce the damage to cotton plants caused by the boll weevil. The present study focuses on the identification of novel molecules that show improved toxicity against the cotton boll weevil. In vitro directed molecular evolution through DNA shuffling and phage display screening was applied to enhance the insecticidal activity of variants of the Cry8Ka1 protein of Bacillus thuringiensis. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Japan | 1 | 1% |
Spain | 1 | 1% |
Italy | 1 | 1% |
France | 1 | 1% |
Unknown | 67 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 18 | 25% |
Student > Master | 11 | 15% |
Professor | 5 | 7% |
Student > Doctoral Student | 4 | 6% |
Student > Postgraduate | 4 | 6% |
Other | 12 | 17% |
Unknown | 17 | 24% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 36 | 51% |
Biochemistry, Genetics and Molecular Biology | 12 | 17% |
Psychology | 2 | 3% |
Environmental Science | 1 | 1% |
Immunology and Microbiology | 1 | 1% |
Other | 1 | 1% |
Unknown | 18 | 25% |