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Environmental Responses in Plants

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Cover of 'Environmental Responses in Plants'

Table of Contents

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    Book Overview
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    Chapter 1 Hydrotropism: Analysis of the Root Response to a Moisture Gradient
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    Chapter 2 Assessing Gravitropic Responses in Arabidopsis
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    Chapter 3 Physiological Analysis of Phototropic Responses in Arabidopsis.
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    Chapter 4 Automatic Chloroplast Movement Analysis.
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    Chapter 5 Microscopic and Biochemical Visualization of Auxins in Plant Tissues.
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    Chapter 6 Immunolocalization of PIN and ABCB Transporters in Plants
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    Chapter 7 Analysis of Circadian Leaf Movements
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    Chapter 8 Sample Preparation of Arabidopsis thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes.
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    Chapter 9 A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs Are Temporally Restricted by the Circadian Clock.
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    Chapter 10 Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hybrid Screens Using a Transcription Factor ORFeome
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    Chapter 11 Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes.
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    Chapter 12 Assessing the Impact of Photosynthetic Sugars on the Arabidopsis Circadian Clock.
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    Chapter 13 Assessing Protein Stability Under Different Light and Circadian Conditions.
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    Chapter 14 Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought.
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    Chapter 15 Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis thaliana.
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    Chapter 16 Assessing Tolerance to Heavy-Metal Stress in Arabidopsis thaliana Seedlings.
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    Chapter 17 Assessing Drought Responses Using Thermal Infrared Imaging.
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    Chapter 18 Generating Targeted Gene Knockout Lines in Physcomitrella patens to Study Evolution of Stress-Responsive Mechanisms.
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    Chapter 19 Screening Stress Tolerance Traits in Arabidopsis Cell Cultures.
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    Chapter 20 Using Arabidopsis Protoplasts to Study Cellular Responses to Environmental Stress.
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    Chapter 21 Construction of Artificial miRNAs to Prevent Drought Stress in Solanum tuberosum.
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    Chapter 22 Virus-Induced Gene Silencing for Gene Function Studies in Barley.
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    Chapter 23 Methods for Long-Term Stable Storage of Colletotrichum Species.
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    Chapter 24 Plant Inoculation with the Fungal Leaf Pathogen Colletotrichum higginsianum.
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    Chapter 25 Tracing Plant Defense Responses in Roots upon MAMP/DAMP Treatment. - PubMed - NCBI
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    Chapter 26 Analysis of the lmmunity-Related Oxidative Bursts by a Luminol-Based Assay
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    Chapter 27 Quantitative Analysis of Microbe-Associated Molecular Pattern (MAMP)-Induced Ca2+ Transients in Plants
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    Chapter 28 Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR
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    Chapter 29 Determining Nucleosome Position at Individual Loci After Biotic Stress Using MNase-qPCR
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    Chapter 30 Phosphoprotein Enrichment Combined with Phosphopeptide Enrichment to Identify Putative Phosphoproteins During Defense Response in Arabidopsis thaliana
Attention for Chapter 25: Tracing Plant Defense Responses in Roots upon MAMP/DAMP Treatment. - PubMed - NCBI
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Chapter title
Tracing Plant Defense Responses in Roots upon MAMP/DAMP Treatment. - PubMed - NCBI
Chapter number 25
Book title
Environmental Responses in Plants
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3356-3_25
Pubmed ID
Book ISBNs
978-1-4939-3354-9, 978-1-4939-3356-3
Authors

Hiruma, Kei, Saijo, Yusuke, Kei Hiruma, Yusuke Saijo

Editors

Paula Duque

Abstract

This chapter describes how to apply microbe-associated molecular pattern (MAMP) or damage-associated molecular pattern (DAMP) solutions to Arabidopsis roots to trace defense responses in the root. Plants sense the presence of microbes via the perception of MAMPs or DAMPs by surface-localized pattern recognition receptors. The mechanisms governing plant root immunity are poorly characterized compared with those underlying plant immunity in the leaf, despite the fact that plant roots constantly interact with countless microbes living in soils that carry potential MAMPs and could stimulate the production of plant-derived DAMPs during colonization. To understand how a plant root immune system detects and reacts to the potential sources of a stimulus, we describe a simple method to monitor activation of root immunity upon MAMP/DAMP treatment by measuring relative expression of defense-related genes by RT-qPCR.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 40%
Researcher 2 40%
Professor > Associate Professor 1 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 60%
Biochemistry, Genetics and Molecular Biology 2 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2016.
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#20,306,690
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Outputs from Methods in molecular biology
#9,917
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Outputs of similar age from Methods in molecular biology
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