| Chapter title |
Analysis of Circadian Leaf Movements
|
|---|---|
| Chapter number | 7 |
| Book title |
Environmental Responses in Plants
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3356-3_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3354-9, 978-1-4939-3356-3
|
| Authors |
Niels A. Müller, José M. Jiménez-Gómez, Müller, Niels A., Jiménez-Gómez, José M. |
| Editors |
Paula Duque |
| Abstract |
The circadian clock is a molecular timekeeper that controls a wide variety of biological processes. In plants, clock outputs range from the molecular level, with rhythmic gene expression and metabolite content, to physiological processes such as stomatal conductance or leaf movements. Any of these outputs can be used as markers to monitor the state of the circadian clock. In the model plant Arabidopsis thaliana, much of the current knowledge about the clock has been gained from time course experiments profiling expression of endogenous genes or reporter constructs regulated by the circadian clock. Since these methods require labor-intensive sample preparation or transformation, monitoring leaf movements is an interesting alternative, especially in non-model species and for natural variation studies. Technological improvements both in digital photography and image analysis allow cheap and easy monitoring of circadian leaf movements. In this chapter we present a protocol that uses an autonomous point and shoot camera and free software to monitor circadian leaf movements in tomato. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Spain | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 11% |
| Unknown | 8 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 33% |
| Student > Bachelor | 2 | 22% |
| Student > Ph. D. Student | 2 | 22% |
| Other | 1 | 11% |
| Professor | 1 | 11% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 56% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |
| Computer Science | 1 | 11% |
| Medicine and Dentistry | 1 | 11% |
| Unknown | 1 | 11% |