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Environmental Responses in Plants

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Cover of 'Environmental Responses in Plants'

Table of Contents

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    Book Overview
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    Chapter 1 Hydrotropism: Analysis of the Root Response to a Moisture Gradient
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    Chapter 2 Assessing Gravitropic Responses in Arabidopsis
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    Chapter 3 Physiological Analysis of Phototropic Responses in Arabidopsis.
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    Chapter 4 Automatic Chloroplast Movement Analysis.
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    Chapter 5 Microscopic and Biochemical Visualization of Auxins in Plant Tissues.
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    Chapter 6 Immunolocalization of PIN and ABCB Transporters in Plants
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    Chapter 7 Analysis of Circadian Leaf Movements
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    Chapter 8 Sample Preparation of Arabidopsis thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes.
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    Chapter 9 A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs Are Temporally Restricted by the Circadian Clock.
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    Chapter 10 Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hybrid Screens Using a Transcription Factor ORFeome
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    Chapter 11 Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes.
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    Chapter 12 Assessing the Impact of Photosynthetic Sugars on the Arabidopsis Circadian Clock.
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    Chapter 13 Assessing Protein Stability Under Different Light and Circadian Conditions.
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    Chapter 14 Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought.
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    Chapter 15 Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis thaliana.
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    Chapter 16 Assessing Tolerance to Heavy-Metal Stress in Arabidopsis thaliana Seedlings.
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    Chapter 17 Assessing Drought Responses Using Thermal Infrared Imaging.
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    Chapter 18 Generating Targeted Gene Knockout Lines in Physcomitrella patens to Study Evolution of Stress-Responsive Mechanisms.
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    Chapter 19 Screening Stress Tolerance Traits in Arabidopsis Cell Cultures.
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    Chapter 20 Using Arabidopsis Protoplasts to Study Cellular Responses to Environmental Stress.
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    Chapter 21 Construction of Artificial miRNAs to Prevent Drought Stress in Solanum tuberosum.
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    Chapter 22 Virus-Induced Gene Silencing for Gene Function Studies in Barley.
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    Chapter 23 Methods for Long-Term Stable Storage of Colletotrichum Species.
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    Chapter 24 Plant Inoculation with the Fungal Leaf Pathogen Colletotrichum higginsianum.
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    Chapter 25 Tracing Plant Defense Responses in Roots upon MAMP/DAMP Treatment. - PubMed - NCBI
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    Chapter 26 Analysis of the lmmunity-Related Oxidative Bursts by a Luminol-Based Assay
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    Chapter 27 Quantitative Analysis of Microbe-Associated Molecular Pattern (MAMP)-Induced Ca2+ Transients in Plants
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    Chapter 28 Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR
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    Chapter 29 Determining Nucleosome Position at Individual Loci After Biotic Stress Using MNase-qPCR
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    Chapter 30 Phosphoprotein Enrichment Combined with Phosphopeptide Enrichment to Identify Putative Phosphoproteins During Defense Response in Arabidopsis thaliana
Attention for Chapter 15: Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis thaliana.
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Chapter title
Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis thaliana.
Chapter number 15
Book title
Environmental Responses in Plants
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3356-3_15
Pubmed ID
Book ISBNs
978-1-4939-3354-9, 978-1-4939-3356-3
Authors

Urszula Piskurewicz, Luis Lopez-Molina

Editors

Paula Duque

Abstract

The model organism Arabidopsis thaliana has been extensively used to unmask the molecular genetic signaling pathways controlling seed germination in plants. In Arabidopsis, the normal seed to seedling developmental transition involves testa rupture soon followed by endosperm rupture, radicle elongation, root hair formation, cotyledon expansion, and greening. Here we detail a number of basic procedures to assess Arabidopsis seed germination in response to different light (red and far-red pulses), temperature (seed thermoinhibition), and water potential (osmotic stress) environmental conditions. We also discuss the role of the endosperm and how its germination-repressive activity can be monitored genetically by means of a seed coat bedding assay. Finally we detail how to evaluate germination responses to changes in gibberellin (GA) and abscisic acid (ABA) levels by manipulating pharmacologically the germination medium.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 41%
Student > Master 5 29%
Student > Bachelor 2 12%
Student > Ph. D. Student 1 6%
Unknown 2 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 47%
Biochemistry, Genetics and Molecular Biology 6 35%
Chemical Engineering 1 6%
Unknown 2 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 June 2016.
All research outputs
#20,333,181
of 22,877,793 outputs
Outputs from Methods in molecular biology
#9,918
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Outputs of similar age
#330,742
of 393,697 outputs
Outputs of similar age from Methods in molecular biology
#1,054
of 1,471 outputs
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So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.