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Environmental Responses in Plants

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Cover of 'Environmental Responses in Plants'

Table of Contents

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    Book Overview
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    Chapter 1 Hydrotropism: Analysis of the Root Response to a Moisture Gradient
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    Chapter 2 Assessing Gravitropic Responses in Arabidopsis
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    Chapter 3 Physiological Analysis of Phototropic Responses in Arabidopsis.
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    Chapter 4 Automatic Chloroplast Movement Analysis.
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    Chapter 5 Microscopic and Biochemical Visualization of Auxins in Plant Tissues.
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    Chapter 6 Immunolocalization of PIN and ABCB Transporters in Plants
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    Chapter 7 Analysis of Circadian Leaf Movements
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    Chapter 8 Sample Preparation of Arabidopsis thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes.
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    Chapter 9 A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs Are Temporally Restricted by the Circadian Clock.
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    Chapter 10 Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hybrid Screens Using a Transcription Factor ORFeome
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    Chapter 11 Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes.
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    Chapter 12 Assessing the Impact of Photosynthetic Sugars on the Arabidopsis Circadian Clock.
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    Chapter 13 Assessing Protein Stability Under Different Light and Circadian Conditions.
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    Chapter 14 Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought.
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    Chapter 15 Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis thaliana.
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    Chapter 16 Assessing Tolerance to Heavy-Metal Stress in Arabidopsis thaliana Seedlings.
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    Chapter 17 Assessing Drought Responses Using Thermal Infrared Imaging.
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    Chapter 18 Generating Targeted Gene Knockout Lines in Physcomitrella patens to Study Evolution of Stress-Responsive Mechanisms.
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    Chapter 19 Screening Stress Tolerance Traits in Arabidopsis Cell Cultures.
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    Chapter 20 Using Arabidopsis Protoplasts to Study Cellular Responses to Environmental Stress.
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    Chapter 21 Construction of Artificial miRNAs to Prevent Drought Stress in Solanum tuberosum.
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    Chapter 22 Virus-Induced Gene Silencing for Gene Function Studies in Barley.
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    Chapter 23 Methods for Long-Term Stable Storage of Colletotrichum Species.
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    Chapter 24 Plant Inoculation with the Fungal Leaf Pathogen Colletotrichum higginsianum.
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    Chapter 25 Tracing Plant Defense Responses in Roots upon MAMP/DAMP Treatment. - PubMed - NCBI
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    Chapter 26 Analysis of the lmmunity-Related Oxidative Bursts by a Luminol-Based Assay
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    Chapter 27 Quantitative Analysis of Microbe-Associated Molecular Pattern (MAMP)-Induced Ca2+ Transients in Plants
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    Chapter 28 Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR
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    Chapter 29 Determining Nucleosome Position at Individual Loci After Biotic Stress Using MNase-qPCR
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    Chapter 30 Phosphoprotein Enrichment Combined with Phosphopeptide Enrichment to Identify Putative Phosphoproteins During Defense Response in Arabidopsis thaliana
Attention for Chapter 11: Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes.
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Chapter title
Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes.
Chapter number 11
Book title
Environmental Responses in Plants
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3356-3_11
Pubmed ID
Book ISBNs
978-1-4939-3354-9, 978-1-4939-3356-3
Authors

Craig G. Simpson, John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, Dorothee Staiger

Editors

Paula Duque

Abstract

Posttranscriptional control makes an important contribution to circadian regulation of gene expression. In higher plants, alternative splicing is particularly prevalent upon abiotic and biotic stress and in the circadian system. Here we describe in detail a high-resolution reverse transcription-PCR based panel (HR RT-PCR) to monitor alternative splicing events. The use of the panel allows the quantification of changes in the proportion of splice isoforms between different samples, e.g., different time points, different tissues, genotypes, ecotypes, or treatments.

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Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 27%
Researcher 2 18%
Student > Master 2 18%
Student > Doctoral Student 1 9%
Professor 1 9%
Other 2 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 64%
Biochemistry, Genetics and Molecular Biology 3 27%
Immunology and Microbiology 1 9%