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Mendeley readers
Chapter title |
Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes.
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Chapter number | 11 |
Book title |
Environmental Responses in Plants
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3356-3_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3354-9, 978-1-4939-3356-3
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Authors |
Craig G. Simpson, John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, Dorothee Staiger |
Editors |
Paula Duque |
Abstract |
Posttranscriptional control makes an important contribution to circadian regulation of gene expression. In higher plants, alternative splicing is particularly prevalent upon abiotic and biotic stress and in the circadian system. Here we describe in detail a high-resolution reverse transcription-PCR based panel (HR RT-PCR) to monitor alternative splicing events. The use of the panel allows the quantification of changes in the proportion of splice isoforms between different samples, e.g., different time points, different tissues, genotypes, ecotypes, or treatments. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 27% |
Researcher | 2 | 18% |
Student > Master | 2 | 18% |
Student > Doctoral Student | 1 | 9% |
Professor | 1 | 9% |
Other | 2 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 64% |
Biochemistry, Genetics and Molecular Biology | 3 | 27% |
Immunology and Microbiology | 1 | 9% |