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Mendeley readers
Chapter title |
Generating Targeted Gene Knockout Lines in Physcomitrella patens to Study Evolution of Stress-Responsive Mechanisms.
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Chapter number | 18 |
Book title |
Environmental Responses in Plants
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3356-3_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3354-9, 978-1-4939-3356-3
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Authors |
Monika Maronova, Maria Kalyna |
Editors |
Paula Duque |
Abstract |
The moss Physcomitrella patens possesses highly efficient homologous recombination allowing targeted gene manipulations and displays many features of the early land plants including high tolerance to abiotic stresses. It is therefore an invaluable model organism for studies of gene functions and comparative studies of evolution of stress responses in plants. Here, we describe a method for generating targeted gene knockout lines in P. patens using a polyethylene glycol-mediated transformation of protoplasts including basic in vitro growth, propagation, and maintenance techniques. |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 5% |
Unknown | 21 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 6 | 27% |
Researcher | 5 | 23% |
Other | 2 | 9% |
Student > Postgraduate | 2 | 9% |
Student > Master | 1 | 5% |
Other | 1 | 5% |
Unknown | 5 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 32% |
Biochemistry, Genetics and Molecular Biology | 6 | 27% |
Environmental Science | 1 | 5% |
Computer Science | 1 | 5% |
Immunology and Microbiology | 1 | 5% |
Other | 1 | 5% |
Unknown | 5 | 23% |