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Variable expressivity of the tumour suppressor protein TRP53 in cryopreserved human blastocysts

Overview of attention for article published in Reproductive Biology and Endocrinology, January 2007
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Title
Variable expressivity of the tumour suppressor protein TRP53 in cryopreserved human blastocysts
Published in
Reproductive Biology and Endocrinology, January 2007
DOI 10.1186/1477-7827-5-39
Pubmed ID
Authors

Vashe Chandrakanthan, Omar Chami, Tomas Stojanov, Chris O'Neill

Abstract

In a mouse model, in vitro fertilization or extended embryo culture leads to the increased expression of TRP53 in susceptible embryos. Ablation of the TRP53 gene improved embryo viability indicating that increased expression of TRP53 is a cause of the reduction of embryo viability resulting from in vitro fertilization or embryo culture. This study investigates the status of TRP53 expression in human embryos produced by intracytoplasmic sperm injection. Following fertilization, embryos were cultured for 96 h and then cryopreserved. Immediately upon thawing they were fixed in formaldehyde and subjected to immunostaining for TRP53. Staining was visualized by confocal microscopy. Negative controls were incubated with isotype control immunoglobulin and showed negligible staining. All embryos showed TRP53 staining above negative controls. TRP53 staining was heterogenous within and between embryos. An embryo that showed retarded development showed high levels of TRP53 expression. A blastocyst that had a collapsed blastocoel also showed high levels of TRP53 compared to morphologically normal blastocysts. Most TRP53 staining was in the region of the nucleus. Morphologically normal blastocysts tended to show little nuclear accumulation of stain. However, some cells within these embryos had high levels of nuclear TRP53 expression. The results show that embryos have varying sensitivity to the stresses of production and culture in vitro, and this resulted in variable expressivity of TRP53.

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 17%
Unknown 5 83%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 50%
Student > Master 1 17%
Professor 1 17%
Unspecified 1 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 50%
Biochemistry, Genetics and Molecular Biology 1 17%
Unspecified 1 17%
Medicine and Dentistry 1 17%