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NF-kappa B

Overview of attention for book
Cover of 'NF-kappa B'

Table of Contents

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    Book Overview
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    Chapter 1 Electrophoretic Mobility Shift Assay Analysis of NF-κB DNA Binding.
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    Chapter 2 Detection of IκB Degradation Dynamics and IκB-α Ubiquitination.
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    Chapter 3 Measurement of NF-κB Transcriptional Activity and Identification of NF-κB cis-Regulatory Elements Using Luciferase Assays.
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    Chapter 4 Assessing Sites of NF-κB DNA Binding Using Chromatin Immunoprecipitation.
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    Chapter 5 IKK Kinase Assay for Assessment of Canonical NF-κB Activation in Neurons.
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    Chapter 6 Analysis of Epidermal Growth Factor-Induced NF-κB Signaling.
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    Chapter 7 Methods to Assess the Activation of the Alternative (Noncanonical) NF-κB Pathway by Non-death TNF Receptors.
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    Chapter 8 Systematic Detection of Noncanonical NF-κB Activation.
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    Chapter 9 Noncanonical NF-κB Activation and SDF-1 Expression in Human Endothelial Cells.
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    Chapter 10 Stable Reconstitution of IKK-Deficient Mouse Embryonic Fibroblasts.
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    Chapter 11 Dissecting NF-κB Signaling Induced by Genotoxic Agents via Genetic Complementation of NEMO-Deficient 1.3E2 Cells.
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    Chapter 12 Visualizing TCR-Induced POLKADOTS Formation and NF-κB Activation in the D10 T-Cell Clone and Mouse Primary Effector T Cells.
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    Chapter 13 Detection of Recombinant and Cellular MALT1 Paracaspase Activity.
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    Chapter 14 TRAF Protein Function in Noncanonical NF-κB Signaling.
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    Chapter 15 Roles of c-IAP Proteins in TNF Receptor Family Activation of NF-κB Signaling.
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    Chapter 16 Elucidating Dynamic Protein-Protein Interactions and Ubiquitination in NF-κB Signaling Pathways.
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    Chapter 17 Immunoblot Analysis of Linear Polyubiquitination of NEMO.
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    Chapter 18 In Vitro Detection of NEMO–Ubiquitin Binding Using DELFIA and Microscale Thermophoresis Assays
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    Chapter 19 Use of Fluorescence Spectroscopy for Quantitative Investigations of Ubiquitin Interactions with the Ubiquitin-Binding Domains of NEMO.
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    Chapter 20 Generation of a Proteolytic Signal: E3/E2-Mediated Polyubiquitination of IκBα.
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    Chapter 21 Control of NF-κB Subunits by Ubiquitination.
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    Chapter 22 Methodology to Study NF-κB/RelA Ubiquitination In Vivo.
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    Chapter 23 Using Sequential Immunoprecipitation and Mass Spectrometry to Identify Methylation of NF-κB.
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    Chapter 24 Methods to Detect NF-κB Acetylation and Methylation.
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    Chapter 25 A Method for the Quantitative Analysis of Stimulation-Induced Nuclear Translocation of the p65 Subunit of NF-κB from Patient-Derived Dermal Fibroblasts.
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    Chapter 26 Methods for Assessing the In Vitro Transforming Activity of NF-κB Transcription Factor c-Rel and Related Proteins.
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    Chapter 27 Using RNA Interference in Lung Cancer Cells to Target the IKK-NF-κB Pathway.
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    Chapter 28 Immunohistochemical Analysis of NF-κB in Human Tumor Tissue.
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    Chapter 29 Assessment of Canonical NF-κB Activity in Canine Diffuse Large B-Cell Lymphoma.
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    Chapter 30 NEMO-Binding Domain Peptide Inhibition of Inflammatory Signal-Induced NF-κB Activation In Vivo.
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    Chapter 31 Regulation of NF-κB Signaling in Osteoclasts and Myeloid Progenitors.
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    Chapter 32 NF-κB Activation with Aging: Characterization and Therapeutic Inhibition.
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    Chapter 33 The "Sneaking-Ligand" Approach: Cell-Type Specific Inhibition of the Classical NF-κB Pathway.
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    Chapter 34 Sneaking-Ligand Fusion Proteins Attenuate Serum Transfer Arthritis by Endothelium-Targeted NF-κB Inhibition.
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    Chapter 35 Analysis of NF-κB Activation in Mouse Intestinal Epithelial Cells.
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    Chapter 36 Characterizing the DNA Binding Site Specificity of NF-κB with Protein-Binding Microarrays (PBMs).
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    Chapter 37 Methods for Analyzing the Evolutionary Relationship of NF-κB Proteins Using Free, Web-Driven Bioinformatics and Phylogenetic Tools.
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    Chapter 38 Studying NF-κB Signaling with Mathematical Models.
Attention for Chapter 35: Analysis of NF-κB Activation in Mouse Intestinal Epithelial Cells.
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Chapter title
Analysis of NF-κB Activation in Mouse Intestinal Epithelial Cells.
Chapter number 35
Book title
NF-kappa B
Published in
Methods in molecular biology, February 2015
DOI 10.1007/978-1-4939-2422-6_35
Pubmed ID
25736774
Book ISBNs
978-1-4939-2421-9, 978-1-4939-2422-6
Authors

Helena Shaked, Monica Guma, Michael Karin, Shaked, Helena, Guma, Monica, Karin, Michael

Editors

Michael J. May

Abstract

Nuclear factor kappa B (NF-κB) is a key transcription factor controlling inflammation, innate immunity, and tissue integrity. NF-κB is activated by IκB kinase (IKK) in response to pro-inflammatory stimuli but is also found to be chronically activated in many inflammatory diseases accompanied by tissue destruction. To study the effects of chronic NF-κB activation in intestinal epithelium, we generated IKKβ(EE)(IEC) transgenic mice which express constitutively active form of IKKβ in their intestinal epithelial cells (IEC). In this chapter, we describe three different methods that we applied for analysis of NF-κB activation in IEC of IKKβ(EE)(IEC) transgenic mice: immunohistochemistry (IHC), nuclear fractionation, and chromatin immunoprecipitation (ChIP). These methods can be also applied to analyze NF-κB activation in mouse intestinal tissue in general.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 33%
Professor > Associate Professor 1 33%
Other 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Immunology and Microbiology 1 33%
Medicine and Dentistry 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2015.
All research outputs
#18,401,956
of 22,793,427 outputs
Outputs from Methods in molecular biology
#7,903
of 13,110 outputs
Outputs of similar age
#256,774
of 352,554 outputs
Outputs of similar age from Methods in molecular biology
#471
of 992 outputs
Altmetric has tracked 22,793,427 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,110 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 992 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.

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