Chapter title |
Methodology to Study NF-κB/RelA Ubiquitination In Vivo.
|
---|---|
Chapter number | 22 |
Book title |
NF-kappa B
|
Published in |
Methods in molecular biology, February 2015
|
DOI | 10.1007/978-1-4939-2422-6_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2421-9, 978-1-4939-2422-6
|
Authors |
Haiying Li, Petro Starokadomskyy, Ezra Burstein, Li, Haiying, Starokadomskyy, Petro, Burstein, Ezra |
Editors |
Michael J. May |
Abstract |
Nuclear factor-kappa B (NF-κB) is a family of transcription factors that regulate immune responses, cell proliferation, differentiation, and survival. Activity of the NF-κΒ pathway on a cellular level is tightly controlled through various mechanisms, one of which is the ubiquitin-dependent degradation of chromatin-bound NF-κB subunits. In general, the ubiquitination of NF-κB regulates the duration of gene transcription activated in response to inflammatory signals. In this article, we present protocols to examine the in vivo ubiquitination status of RelA, a critical protein of the NF-κB family. |
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