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Lactogenic hormones alter cellular and extracellular microRNA expression in bovine mammary epithelial cell culture

Overview of attention for article published in Journal of Animal Science and Biotechnology, February 2016
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3 tweeters

Citations

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19 Dimensions

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24 Mendeley
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Title
Lactogenic hormones alter cellular and extracellular microRNA expression in bovine mammary epithelial cell culture
Published in
Journal of Animal Science and Biotechnology, February 2016
DOI 10.1186/s40104-016-0068-x
Pubmed ID
Authors

Susumu Muroya, Tatsuro Hagi, Ataru Kimura, Hisashi Aso, Masatoshi Matsuzaki, Masaru Nomura

Abstract

Bovine milk contains not only a variety of nutritional ingredients but also microRNAs (miRNAs) that are thought to be secreted by the bovine mammary epithelial cells (BMECs). The objective of this study was to elucidate the production of milk-related miRNAs in BMECs under the influence of lactogenic hormones. According to a microarray result of milk exosomal miRNAs prior to cellular analyses, a total of 257 miRNAs were detected in a Holstein cow milk. Of these, 18 major miRNAs of interest in the milk were selected for an expression analysis in BMEC culture that was treated with or without dexamethasone, insulin, and prolactin (DIP) to induce a lactogenic differentiation. Quantitative polymerase chain reaction (qPCR) results showed that the expressions of miR-21-5p (P = 0.005), miR-26a (P = 0.016), and miR-320a (P = 0.011) were lower in the DIP-treated cells than in the untreated cells. In contrast, the expression of miR-339a (P = 0.017) in the cell culture medium were lower in the DIP-treated culture than in the untreated culture. Intriguingly, the miR-148a expression in cell culture medium was elevated by DIP treatment of BMEC culture (P = 0.018). The medium-to-cell expression ratios of miR-103 (P = 0.025), miR-148a (P < 0.001), and miR-223 (P = 0.013) were elevated in the DIP-treated BMECs, suggesting that the lactogenic differentiation-induced secretion of these three miRNAs in BMECs. A bioinformatic analysis showed that the miRNAs down-regulated in the BMECs were associated with the suppression of genes related to transcriptional regulation, protein phosphorylation, and tube development. The results suggest that the miRNAs changed by lactogenic hormones are associated with milk protein synthesis, and mammary gland development and maturation. The elevated miR-148a level in DIP-treated BMECs may be associated with its increase in milk during the lactation period of cows.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 17%
Student > Master 4 17%
Student > Ph. D. Student 4 17%
Other 3 13%
Student > Bachelor 2 8%
Other 5 21%
Unknown 2 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 54%
Biochemistry, Genetics and Molecular Biology 4 17%
Veterinary Science and Veterinary Medicine 1 4%
Business, Management and Accounting 1 4%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Other 1 4%
Unknown 3 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2016.
All research outputs
#7,205,442
of 12,554,960 outputs
Outputs from Journal of Animal Science and Biotechnology
#82
of 273 outputs
Outputs of similar age
#120,323
of 267,032 outputs
Outputs of similar age from Journal of Animal Science and Biotechnology
#4
of 6 outputs
Altmetric has tracked 12,554,960 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 273 research outputs from this source. They receive a mean Attention Score of 2.5. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,032 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.