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Phylogenetic analysis of Dengue virus 1 isolated from South Minas Gerais, Brazil

Overview of attention for article published in Brazilian Journal of Microbiology, January 2016
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Title
Phylogenetic analysis of Dengue virus 1 isolated from South Minas Gerais, Brazil
Published in
Brazilian Journal of Microbiology, January 2016
DOI 10.1016/j.bjm.2015.11.016
Pubmed ID
Authors

Betania Paiva Drumond, Luiz Gustavo da Silva Fagundes, Raissa Prado Rocha, Marcilio Jorge Fumagalli, Carlos Shigueru Araki, Tatiana Elisa Colombo, Mauricio Lacerda Nogueira, Thiago Elias Castilho, Nelson José Freitas da Silveira, Luiz Cosme Cotta Malaquias, Luiz Felipe Leomil Coelho

Abstract

Dengue is a major worldwide public health problem, especially in the tropical and subtropical regions of the world. Primary infection with a single Dengue virus serotype causes a mild, self-limiting febrile illness called dengue fever. However, a subset of patients who experience secondary infection with a different serotype can progress to a more severe form of the disease, called dengue hemorrhagic fever. The four Dengue virus serotypes (1-4) are antigenically and genetically distinct and each serotype is composed of multiple genotypes. In this study we isolated one Dengue virus 1 serotype, named BR/Alfenas/2012, from a patient with dengue hemorrhagic fever in Alfenas, South Minas Gerais, Brazil and molecular identification was performed based on the analysis of NS5 gene. Swiss mice were infected with this isolate to verify its potential to induce histopathological alterations characteristic of dengue. Liver histopathological analysis of infected animals showed the presence of inflammatory infiltrates, hepatic steatosis, as well as edema, hemorrhage and necrosis focal points. Phylogenetic and evolutionary analyses based on the envelope gene provided evidence that the isolate BR/Alfenas/2012 belongs to genotype V, lineage I and it is probably derived from isolates of Rio de Janeiro, Brazil. The isolate BR/Alfenas/2012 showed two unique amino acids substitutions (SER222THRE and PHE306SER) when compared to other Brazilian isolates from the same genotype/lineage. Molecular models were generated for the envelope protein indicating that the amino acid alteration PHE 306 SER could contribute to a different folding in this region located within the domain III. Further genetic and animal model studies using BR/Alfenas/2012 and other isolates belonging to the same lineage/genotype could help determine the relation of these genetic alterations and dengue hemorrhagic fever in a susceptible population.

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Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 2 4%
Brazil 2 4%
Unknown 48 92%

Demographic breakdown

Readers by professional status Count As %
Student > Master 9 17%
Student > Bachelor 8 15%
Researcher 8 15%
Student > Ph. D. Student 7 13%
Student > Doctoral Student 3 6%
Other 9 17%
Unknown 8 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 25%
Biochemistry, Genetics and Molecular Biology 8 15%
Medicine and Dentistry 5 10%
Immunology and Microbiology 4 8%
Veterinary Science and Veterinary Medicine 2 4%
Other 7 13%
Unknown 13 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 February 2016.
All research outputs
#9,893,104
of 12,355,257 outputs
Outputs from Brazilian Journal of Microbiology
#243
of 363 outputs
Outputs of similar age
#195,333
of 279,250 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#5
of 8 outputs
Altmetric has tracked 12,355,257 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 363 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 22nd percentile – i.e., 22% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,250 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.