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Characterization and identification of the xylanolytic enzymes from Aspergillus fumigatus Z5

Overview of attention for article published in BMC Microbiology, June 2015
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (56th percentile)
  • Good Attention Score compared to outputs of the same age and source (66th percentile)

Mentioned by

patent
1 patent

Citations

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27 Dimensions

Readers on

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50 Mendeley
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Title
Characterization and identification of the xylanolytic enzymes from Aspergillus fumigatus Z5
Published in
BMC Microbiology, June 2015
DOI 10.1186/s12866-015-0463-z
Pubmed ID
Authors

Youzhi Miao, Juan Li, Zhizhuang Xiao, Qirong Shen, Ruifu Zhang

Abstract

Plant biomass, the most abundant natural material on earth, represents a vast source of food and energy in nature. As the main component of plant biomass, xylan is a complex polysaccharide comprising a linear β(1,4)-linked backbone of xylosyl residues substituted by acetyl, arabinosyl, glucuronysyl and 4-O-methylglucuronycyl residues. Aspergillus fumigatus Z5 is an efficient plant biomass depolymerization fungus. In this study, its crude xylanolytic enzymes were characterized and identified by two-dimensional gel electrophoresis (2-DE). The optimal temperature for the crude xylanases was close to 60 °C, the highest xylanase activity was achieved at pH ranged from 3 to 6, and the crude xylanases also showed a very broad region of pH (3-11) stability. The maximal xylanase activity of 21.45 U · ml(-1) was observed in the fourth day of cultivation at 50 °C and 150 rpm with 2 % xylan as the sole carbon source. Zymogram analysis indicated that there were more than seven secreted proteins with xylanase activity. In the crude enzyme, two major endoxylanases, five cellulases and several associated enzymes were identified to be involved in the hydrolysis of polysaccharides. Of the total 13 xylanase genes in the Z5 genome, 11 were observed using q-PCR to be induced by xylan, one of which, An endo-1,4-β-xylanase with a low secretion level, was also expressed and characterized. The final hydrolysis products of xylan by crude enzyme mainly consisted of xylobiose. This study provides a comprehensive understanding of the depolymerization of xylan by Z5 and will help to design enzymatic strategies for plant biomass utilization.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 50 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Ireland 1 2%
Unknown 49 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 24%
Student > Ph. D. Student 8 16%
Student > Bachelor 4 8%
Researcher 4 8%
Student > Doctoral Student 2 4%
Other 6 12%
Unknown 14 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 15 30%
Agricultural and Biological Sciences 11 22%
Chemical Engineering 2 4%
Medicine and Dentistry 2 4%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Other 2 4%
Unknown 17 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 June 2021.
All research outputs
#7,729,323
of 23,498,099 outputs
Outputs from BMC Microbiology
#884
of 3,256 outputs
Outputs of similar age
#90,596
of 265,366 outputs
Outputs of similar age from BMC Microbiology
#16
of 48 outputs
Altmetric has tracked 23,498,099 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,256 research outputs from this source. They receive a mean Attention Score of 4.2. This one has gotten more attention than average, scoring higher than 65% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,366 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.
We're also able to compare this research output to 48 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.