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Mendeley readers
Attention Score in Context
| Title |
Structure of a VHH isolated from a naïve phage display library
|
|---|---|
| Published in |
BMC Research Notes, March 2019
|
| DOI | 10.1186/s13104-019-4197-0 |
| Pubmed ID | |
| Authors |
Brandy White, Ian Huh, Cory L. Brooks |
| Abstract |
To determine the X-ray structure and biophysical properties of a Camelid VHH isolated from a naïve phage display library. Single domain antibodies (VHH) derived from the unique immune system of the Camelidae family have gained traction as useful tools for biotechnology as well as a source of potentially novel therapeutics. Here we report the structure and biophysical characterization of a VHH originally isolated from a naïve camelid phage display library. VHH R419 has a melting temperate of 66 °C and was found to be a monomer in solution. The protein crystallized in space group P6522 and the structure was solved by molecular replacement to a resolution of 1.5 Å. The structure revealed a flat paratope with CDR loops that could be classified into existing canonical loop structures. A combination of high expression yield, stability and rapid crystallization might make R419 into a candidate scaffold for CDR grafting and homology modeling. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 34 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 21% |
| Student > Ph. D. Student | 5 | 15% |
| Student > Bachelor | 5 | 15% |
| Student > Master | 3 | 9% |
| Other | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 11 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 26% |
| Chemistry | 3 | 9% |
| Agricultural and Biological Sciences | 2 | 6% |
| Veterinary Science and Veterinary Medicine | 2 | 6% |
| Engineering | 2 | 6% |
| Other | 5 | 15% |
| Unknown | 11 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 April 2019.
All research outputs
#18,676,383
of 23,140,503 outputs
Outputs from BMC Research Notes
#3,041
of 4,292 outputs
Outputs of similar age
#266,143
of 351,899 outputs
Outputs of similar age from BMC Research Notes
#61
of 86 outputs
Altmetric has tracked 23,140,503 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,292 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 351,899 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 86 others from the same source and published within six weeks on either side of this one. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.