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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases
|
|---|---|
| Published in |
Cell Reports, February 2016
|
| DOI | 10.1016/j.celrep.2016.02.018 |
| Pubmed ID | |
| Authors |
Jean-Baptiste Renaud, Charlotte Boix, Marine Charpentier, Anne De Cian, Julien Cochennec, Evelyne Duvernois-Berthet, Loïc Perrouault, Laurent Tesson, Joanne Edouard, Reynald Thinard, Yacine Cherifi, Séverine Menoret, Sandra Fontanière, Noémie de Crozé, Alexandre Fraichard, Frédéric Sohm, Ignacio Anegon, Jean-Paul Concordet, Carine Giovannangeli |
| Abstract |
Genome editing has now been reported in many systems using TALEN and CRISPR-Cas9 nucleases. Precise mutations can be introduced during homology-directed repair with donor DNA carrying the wanted sequence edit, but efficiency is usually lower than for gene knockout and optimal strategies have not been extensively investigated. Here, we show that using phosphorothioate-modified oligonucleotides strongly enhances genome editing efficiency of single-stranded oligonucleotide donors in cultured cells. In addition, it provides better design flexibility, allowing insertions more than 100 bp long. Despite previous reports of phosphorothioate-modified oligonucleotide toxicity, clones of edited cells are readily isolated and targeted sequence insertions are achieved in rats and mice with very high frequency, allowing for homozygous loxP site insertion at the mouse ROSA locus in particular. Finally, when detected, imprecise knockin events exhibit indels that are asymmetrically positioned, consistent with genome editing taking place by two steps of single-strand annealing. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 17% |
| United States | 1 | 17% |
| Australia | 1 | 17% |
| Unknown | 3 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 50% |
| Members of the public | 2 | 33% |
| Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 611 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | <1% |
| United States | 3 | <1% |
| France | 2 | <1% |
| Brazil | 1 | <1% |
| Czechia | 1 | <1% |
| Denmark | 1 | <1% |
| Switzerland | 1 | <1% |
| Japan | 1 | <1% |
| Spain | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 597 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 145 | 24% |
| Researcher | 145 | 24% |
| Student > Master | 70 | 11% |
| Student > Bachelor | 53 | 9% |
| Other | 30 | 5% |
| Other | 59 | 10% |
| Unknown | 109 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 244 | 40% |
| Agricultural and Biological Sciences | 162 | 27% |
| Medicine and Dentistry | 23 | 4% |
| Neuroscience | 14 | 2% |
| Immunology and Microbiology | 9 | 1% |
| Other | 35 | 6% |
| Unknown | 124 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2024.
All research outputs
#2,983,331
of 25,374,647 outputs
Outputs from Cell Reports
#6,086
of 12,958 outputs
Outputs of similar age
#44,997
of 312,901 outputs
Outputs of similar age from Cell Reports
#107
of 269 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 12,958 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 30.3. This one has gotten more attention than average, scoring higher than 52% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 312,901 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 269 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.