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Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases

Overview of attention for article published in Cell Reports, February 2016
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (63rd percentile)

Mentioned by

twitter
6 tweeters

Readers on

mendeley
226 Mendeley
citeulike
3 CiteULike
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Title
Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases
Published in
Cell Reports, February 2016
DOI 10.1016/j.celrep.2016.02.018
Pubmed ID
Authors

Jean-Baptiste Renaud, Charlotte Boix, Marine Charpentier, Anne De Cian, Julien Cochennec, Evelyne Duvernois-Berthet, Loïc Perrouault, Laurent Tesson, Joanne Edouard, Reynald Thinard, Yacine Cherifi, Séverine Menoret, Sandra Fontanière, Noémie de Crozé, Alexandre Fraichard, Frédéric Sohm, Ignacio Anegon, Jean-Paul Concordet, Carine Giovannangeli, Renaud, Jean-Baptiste, Boix, Charlotte, Charpentier, Marine, De Cian, Anne, Cochennec, Julien, Duvernois-Berthet, Evelyne, Perrouault, Loïc, Tesson, Laurent, Edouard, Joanne, Thinard, Reynald, Cherifi, Yacine, Menoret, Séverine, Fontanière, Sandra, de Crozé, Noémie, Fraichard, Alexandre, Sohm, Frédéric, Anegon, Ignacio, Concordet, Jean-Paul, Giovannangeli, Carine

Abstract

Genome editing has now been reported in many systems using TALEN and CRISPR-Cas9 nucleases. Precise mutations can be introduced during homology-directed repair with donor DNA carrying the wanted sequence edit, but efficiency is usually lower than for gene knockout and optimal strategies have not been extensively investigated. Here, we show that using phosphorothioate-modified oligonucleotides strongly enhances genome editing efficiency of single-stranded oligonucleotide donors in cultured cells. In addition, it provides better design flexibility, allowing insertions more than 100 bp long. Despite previous reports of phosphorothioate-modified oligonucleotide toxicity, clones of edited cells are readily isolated and targeted sequence insertions are achieved in rats and mice with very high frequency, allowing for homozygous loxP site insertion at the mouse ROSA locus in particular. Finally, when detected, imprecise knockin events exhibit indels that are asymmetrically positioned, consistent with genome editing taking place by two steps of single-strand annealing.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 226 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 3 1%
United States 3 1%
Germany 2 <1%
Switzerland 2 <1%
France 2 <1%
Spain 1 <1%
Denmark 1 <1%
Brazil 1 <1%
Japan 1 <1%
Other 2 <1%
Unknown 208 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 71 31%
Researcher 70 31%
Student > Master 36 16%
Student > Bachelor 14 6%
Other 14 6%
Other 21 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 119 53%
Biochemistry, Genetics and Molecular Biology 76 34%
Medicine and Dentistry 9 4%
Unspecified 6 3%
Neuroscience 6 3%
Other 10 4%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 March 2016.
All research outputs
#2,844,711
of 7,349,306 outputs
Outputs from Cell Reports
#2,381
of 3,431 outputs
Outputs of similar age
#100,374
of 282,802 outputs
Outputs of similar age from Cell Reports
#221
of 296 outputs
Altmetric has tracked 7,349,306 research outputs across all sources so far. This one has received more attention than most of these and is in the 60th percentile.
So far Altmetric has tracked 3,431 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 21.5. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 282,802 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.
We're also able to compare this research output to 296 others from the same source and published within six weeks on either side of this one. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.