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A workflow to process 3D+time microscopy images of developing organisms and reconstruct their cell lineage

Overview of attention for article published in Nature Communications, January 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (55th percentile)

Mentioned by

twitter
32 tweeters

Citations

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25 Dimensions

Readers on

mendeley
96 Mendeley
citeulike
1 CiteULike
Title
A workflow to process 3D+time microscopy images of developing organisms and reconstruct their cell lineage
Published in
Nature Communications, January 2016
DOI 10.1038/ncomms9674
Pubmed ID
Authors

Emmanuel Faure, Thierry Savy, Barbara Rizzi, Camilo Melani, Olga Stašová, Dimitri Fabrèges, Róbert Špir, Mark Hammons, Róbert Čúnderlík, Gaëlle Recher, Benoît Lombardot, Louise Duloquin, Ingrid Colin, Jozef Kollár, Sophie Desnoulez, Pierre Affaticati, Benoît Maury, Adeline Boyreau, Jean-Yves Nief, Pascal Calvat, Philippe Vernier, Monique Frain, Georges Lutfalla, Yannick Kergosien, Pierre Suret, Mariana Remešíková, René Doursat, Alessandro Sarti, Karol Mikula, Nadine Peyriéras, Paul Bourgine

Abstract

The quantitative and systematic analysis of embryonic cell dynamics from in vivo 3D+time image data sets is a major challenge at the forefront of developmental biology. Despite recent breakthroughs in the microscopy imaging of living systems, producing an accurate cell lineage tree for any developing organism remains a difficult task. We present here the BioEmergences workflow integrating all reconstruction steps from image acquisition and processing to the interactive visualization of reconstructed data. Original mathematical methods and algorithms underlie image filtering, nucleus centre detection, nucleus and membrane segmentation, and cell tracking. They are demonstrated on zebrafish, ascidian and sea urchin embryos with stained nuclei and membranes. Subsequent validation and annotations are carried out using Mov-IT, a custom-made graphical interface. Compared with eight other software tools, our workflow achieved the best lineage score. Delivered in standalone or web service mode, BioEmergences and Mov-IT offer a unique set of tools for in silico experimental embryology.

Twitter Demographics

The data shown below were collected from the profiles of 32 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 96 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 2 2%
United States 2 2%
Germany 1 1%
Switzerland 1 1%
Slovakia 1 1%
United Kingdom 1 1%
Japan 1 1%
Netherlands 1 1%
Unknown 86 90%

Demographic breakdown

Readers by professional status Count As %
Researcher 22 23%
Student > Master 18 19%
Student > Ph. D. Student 18 19%
Student > Bachelor 8 8%
Unspecified 7 7%
Other 23 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 42 44%
Biochemistry, Genetics and Molecular Biology 16 17%
Unspecified 9 9%
Computer Science 8 8%
Engineering 6 6%
Other 15 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 February 2017.
All research outputs
#725,925
of 11,793,446 outputs
Outputs from Nature Communications
#8,058
of 18,330 outputs
Outputs of similar age
#27,654
of 292,044 outputs
Outputs of similar age from Nature Communications
#365
of 823 outputs
Altmetric has tracked 11,793,446 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 18,330 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 47.3. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 292,044 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 823 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.