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Microbial Environmental Genomics (MEG)

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Cover of 'Microbial Environmental Genomics (MEG)'

Table of Contents

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    Book Overview
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    Chapter 1 "Deciphering Archaeal Communities" Omics Tools in the Study of Archaeal Communities.
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    Chapter 2 Investigating the Endobacteria Which Thrive in Arbuscular Mycorrhizal Fungi
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    Chapter 3 GenoSol Platform: A Logistic and Technical Platform for Conserving and Exploring Soil Microbial Diversity.
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    Chapter 4 Sample Preparation for Fungal Community Analysis by High-Throughput Sequencing of Barcode Amplicons
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    Chapter 5 Fungal Communities in Soils: Soil Organic Matter Degradation.
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    Chapter 6 DNA-Based Characterization and Identification of Arbuscular Mycorrhizal Fungi Species.
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    Chapter 7 Molecular Identification of Soil Eukaryotes and Focused Approaches Targeting Protist and Faunal Groups Using High-Throughput Metabarcoding.
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    Chapter 8 Identification and In Situ Distribution of a Fungal Gene Marker: The Mating Type Genes of the Black Truffle
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    Chapter 9 Stable-Isotope Probing RNA to Study Plant/Fungus Interactions
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    Chapter 10 Targeted Gene Capture by Hybridization to Illuminate Ecosystem Functioning.
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    Chapter 11 Hybridization of Environmental Microbial Community Nucleic Acids by GeoChip.
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    Chapter 12 Reconstruction of Transformation Processes Catalyzed by the Soil Microbiome Using Metagenomic Approaches.
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    Chapter 13 MG-RAST, a Metagenomics Service for Analysis of Microbial Community Structure and Function.
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    Chapter 14 Analysis of Active Methylotrophic Communities: When DNA-SIP Meets High-Throughput Technologies.
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    Chapter 15 Functional Metagenomics: Construction and High-Throughput Screening of Fosmid Libraries for Discovery of Novel Carbohydrate-Active Enzymes.
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    Chapter 16 Metatranscriptomics of Soil Eukaryotic Communities
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    Chapter 17 Analysis of Ancient DNA in Microbial Ecology.
Attention for Chapter 16: Metatranscriptomics of Soil Eukaryotic Communities
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Chapter title
Metatranscriptomics of Soil Eukaryotic Communities
Chapter number 16
Book title
Microbial Environmental Genomics (MEG)
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3369-3_16
Pubmed ID
Book ISBNs
978-1-4939-3367-9, 978-1-4939-3369-3
Authors

Rajiv K. Yadav, Claudia Bragalini, Laurence Fraissinet-Tachet, Roland Marmeisse, Patricia Luis

Editors

Francis Martin, Stephane Uroz

Abstract

Functions expressed by eukaryotic organisms in soil can be specifically studied by analyzing the pool of eukaryotic-specific polyadenylated mRNA directly extracted from environmental samples. In this chapter, we describe two alternative protocols for the extraction of high-quality RNA from soil samples. Total soil RNA or mRNA can be converted to cDNA for direct high-throughput sequencing. Polyadenylated mRNA-derived full-length cDNAs can also be cloned in expression plasmid vectors to constitute soil cDNA libraries, which can be subsequently screened for functional gene categories. Alternatively, the diversity of specific gene families can also be explored following cDNA sequence capture using exploratory oligonucleotide probes.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 4%
United States 1 4%
France 1 4%
Unknown 25 89%

Demographic breakdown

Readers by professional status Count As %
Student > Master 10 36%
Student > Ph. D. Student 5 18%
Professor > Associate Professor 4 14%
Researcher 4 14%
Professor 1 4%
Other 1 4%
Unknown 3 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 39%
Environmental Science 4 14%
Biochemistry, Genetics and Molecular Biology 2 7%
Immunology and Microbiology 2 7%
Engineering 2 7%
Other 2 7%
Unknown 5 18%