Chapter title |
Functional Metagenomics: Construction and High-Throughput Screening of Fosmid Libraries for Discovery of Novel Carbohydrate-Active Enzymes.
|
---|---|
Chapter number | 15 |
Book title |
Microbial Environmental Genomics (MEG)
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3369-3_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3367-9, 978-1-4939-3369-3
|
Authors |
Ufarté, Lisa, Bozonnet, Sophie, Laville, Elisabeth, Cecchini, Davide A, Pizzut-Serin, Sandra, Jacquiod, Samuel, Demanèche, Sandrine, Simonet, Pascal, Franqueville, Laure, Potocki Veronese, Gabrielle, Lisa Ufarté, Sophie Bozonnet, Elisabeth Laville, Davide A. Cecchini, Sandra Pizzut-Serin, Samuel Jacquiod, Sandrine Demanèche, Pascal Simonet, Laure Franqueville, Gabrielle Potocki Veronese |
Editors |
Francis Martin, Stephane Uroz |
Abstract |
Activity-based metagenomics is one of the most efficient approaches to boost the discovery of novel biocatalysts from the huge reservoir of uncultivated bacteria. In this chapter, we describe a highly generic procedure of metagenomic library construction and high-throughput screening for carbohydrate-active enzymes. Applicable to any bacterial ecosystem, it enables the swift identification of functional enzymes that are highly efficient, alone or acting in synergy, to break down polysaccharides and oligosaccharides. |
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