Targeted Gene Capture by Hybridization to Illuminate Ecosystem Functioning.
Microbial Environmental Genomics (MEG)
Methods in molecular biology, January 2016
Ribière, Céline, Beugnot, Réjane, Parisot, Nicolas, Gasc, Cyrielle, Defois, Clémence, Denonfoux, Jérémie, Boucher, Delphine, Peyretaillade, Eric, Peyret, Pierre, Céline Ribière, Réjane Beugnot, Nicolas Parisot, Cyrielle Gasc, Clémence Defois, Jérémie Denonfoux, Delphine Boucher, Eric Peyretaillade, Pierre Peyret
Francis Martin, Stephane Uroz
Microbial communities are extremely abundant and diverse on earth surface and play key role in the ecosystem functioning. Thus, although next-generation sequencing (NGS) technologies have greatly improved knowledge on microbial diversity, it is necessary to reduce the biological complexity to better understand the microorganism functions. To achieve this goal, we describe a promising approach, based on the solution hybrid selection (SHS) method for the selective enrichment in a target-specific biomarker from metagenomic and metatranscriptomic samples. The success of this method strongly depends on the determination of sensitive, specific, and explorative probes to assess the complete targeted gene repertoire. Indeed, in this method, RNA probes were used to capture large DNA or RNA fragments harboring biomarkers of interest that potentially allow to link structure and function of communities of interest.
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