"Deciphering Archaeal Communities" Omics Tools in the Study of Archaeal Communities.
Microbial Environmental Genomics (MEG)
Methods in molecular biology, January 2016
Pašić, Lejla, Martin-Cuadrado, Ana-Belen, López-García, Purificación, Lejla Pašić, Ana-Belen Martin-Cuadrado, Purificación López-García
Francis Martin, Stephane Uroz
Archaea constitute one of the three recognized phylogenetic groups of organisms living on the planet, and the latest to be discovered. Most Archaea resist cultivation and are studied using molecular methods. High-throughput amplicon sequencing and metagenomic approaches have been key in uncovering hitherto unknown archaeal diversity, their metabolic potential, and have even provided an insight into genomes of a number of uncultivated members of this group. Here, we summarize protocols describing sampling, molecular, metagenomic, and metatranscriptomic analyses as well as bioinformatics approaches that have proved useful for the study of archaea in natural samples.
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