DNA-Based Characterization and Identification of Arbuscular Mycorrhizal Fungi Species.
Microbial Environmental Genomics (MEG)
Methods in molecular biology, January 2016
Senés-Guerrero, Carolina, Schüßler, Arthur, Carolina Senés-Guerrero, Arthur Schüßler
Francis Martin, Stephane Uroz
Arbuscular mycorrhizal fungi (AMF) are obligate symbionts of most land plants. They have great ecological and economic importance as they can improve plant nutrition, plant water supply, soil structure, and plant resistance to pathogens. We describe two approaches for the DNA-based characterization and identification of AMF, which both can be used for single fungal spores, soil, or roots samples and resolve closely related AMF species: (a) Sanger sequencing of a 1.5 kb extended rDNA-barcode from clone libraries, e.g., to characterize AMF isolates, and (b) high throughput 454 GS-FLX+ pyrosequencing of a 0.8 kb rDNA fragment, e.g., for in-field monitoring.
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